黄瓜持绿蛋白与候选分泌效应蛋白互作分析  

Analysis of Interaction between Cucumber Stay Green Protein and Candidate Secreted Effector Protein

作　　者：陈蒙 邹杭宇 刘海峰[2] 李正焕 CHEN Meng;ZOU Hangyu;LIU Haifeng;LEE Jehuan(College of Natural Sciences,Chonbuk National University,Jeonju 54896,South Korea;College of Agriculture,Yanbian University,Yanji 133002,China)

机构地区：[1]韩国全北国立大学自然科学学院,韩国全州54896 [2]延边大学农学院,吉林延吉133002

出　　处：《种业导刊》2025年第2期17-23,共7页Journal of Seed Industry Guide

基　　金：韩国农业发展局(RDA)项目(PJ01596502);韩国全北国立大学BK21四大项目;延边大学创业项目。

摘　　要：采用酵母双杂交技术筛选与黄瓜持绿蛋白(Stay green,SGR)互作的候选分泌效应蛋白(Candidate secreted effector protein,CSEP),并通过GST-pull down和免疫共沉淀技术进行验证。结果表明,克隆获得黄瓜CsSGR和CsCSEP基因。酵母双杂交试验结果表明,CsSGR蛋白与CsCSEP30、CsCSEP47、CsCSEP48蛋白具有交互作用。GST-pull down和免疫共沉淀试验均验证了CsSGR蛋白与CsCSEP30、CsCSEP47、CsCSEP48蛋白的交互作用。综上,CsSGR蛋白与CsCSEP30、CsCSEP47、CsCSEP48蛋白互作,CsSGR蛋白可能有助于减缓叶片的衰老进程并增强抗病能力。The yeast two‑hybrid technology was used to screen candidate secreted effector proteins(CSEP)that interact with cucumber stay green protein(SGR).The interaction was further validated by GST‑pull down and co‑immunoprecipitation techniques.The results showed that the cucumber CsSGR and CsCSEP genes were successfully cloned.The yeast two‑hybrid assay revealed that CsSGR protein interacted with CsCSEP30,CsCSEP47 and CsCSEP48 proteins.Both GST‑pull down and co‑immunoprecipitation assays confirmed the interaction between CsSGR protein and CsCSEP30,CsCSEP47 and CsCSEP48 proteins.In conclusion,CsSGR protein interacts with CsCSEP30,CsCSEP47 and CsCSEP48 proteins,CsSGR may help to delay leaf senescence and enhance disease resistance.

关 键 词：黄瓜 持绿蛋白 候选分泌效应蛋白 蛋白互作 酵母双杂交 免疫共沉淀 

分 类 号：S642[农业科学—蔬菜学]