基于流固耦合方法的微流控芯片内细胞变形研究  

A Study on Cellular Deformation within Microfluidic Chips Utilizing Fluid-Structure Interaction Analysis

作　　者：薛韩晓 朱博婷 毛琳 Hanxiao Xue;Boting Zhu;Lin Mao(Modern Minimally Invasive Medical Devices and Technology ERC,MOE,School of Health Science and Engineering,University of Shanghai for Science and Technology,Shanghai)

机构地区：[1]上海理工大学健康科学与工程学院,现代微创医疗器械及技术教育部工程研究中心,上海

出　　处：《建模与仿真》2025年第3期199-211,共13页Modeling and Simulation

摘　　要：本文采用流固耦合(Fluid-Structure Interaction,FSI)方法,针对微流控芯片内细胞的变形行为进行了深入研究。为克服传统大批量细胞分析方法在捕捉单个细胞细微差异方面的局限性,我们设计了一种新型微流控芯片,专用于单细胞的捕获。利用COMSOL Multiphysics多物理场仿真软件,我们对该芯片进行了精细建模与仿真,系统探讨了其对不同弹性模量单细胞的捕获效能。通过模拟细胞在芯片中的流动与捕获过程,我们详细分析了细胞表面应力分布、液体流速、捕获时间以及细胞旋转角度等关键参数。研究结果显示,该微流控芯片能够高效且无损地捕获具有不同弹性模量的细胞。这一发现不仅证实了芯片设计在单细胞捕获方面的有效性,还为微流控技术在单细胞分析领域的更广泛应用奠定了坚实的理论基础,并提供了有力的技术支持。In this paper,we investigated the deformation behavior of cells within a microfluidic chip based on the Fluid-Structure Interaction(FSI)method.In order to overcome the limitations of traditional bulk cell analysis methods that are difficult to capture the subtle differences of individual cells,a micro-fluidic chip was designed for capturing single cells in this study.The microfluidic chip was modeled and simulated using COMSOL Multiphysics multiphysics field simulation software,and the effect of the chip on the capture of single cells with different elastic moduli was discussed in depth.By simu-lating the cell flow and capture process in the chip,key parameters such as cell surface stress distri-bution,liquid flow rate,capture time and cell rotation angle were analyzed.The results show that the microfluidic chip can effectively capture cells with different elastic moduli and the cells will not be mechanically damaged during the capture process.This finding not only verifies the effectiveness of the microfluidic chip design in capturing single cells efficiently and nondestructively,but also provides a solid theoretical foundation and technical support for the further application of micro-fluidic technology in the field of single cell analysis.

关 键 词：单细胞 微流控 层流 流固耦合 细胞形变 多物理场仿真 

分 类 号：Q2-33[生物学—细胞生物学]