便携式PCR实时光电检测系统设计  

The Design of a Portable PCR Real-Time Optical Detection System

作　　者：徐志凯 瑚琦[1,2] Zhikai Xu;Qi Hu(Shanghai Key Laboratory of Modern Optical Systems,Shanghai;School of Optical-Electrical&Computer Engineering,University of Shanghai for Science&Technology,Shanghai)

机构地区：[1]上海市现代光学系统重点实验室,上海 [2]上海理工大学光电信息与计算机工程学院,上海

出　　处：《建模与仿真》2025年第3期487-499,共13页Modeling and Simulation

摘　　要：针对传统荧光聚合酶链式反应(Polymerase Chain Reaction,PCR)检测仪操作复杂、体积大、成本高的问题,设计了一款便携式实时荧光检测设备。该设备集光机电一体化,集成度高。光学系统基于Zemax光学设计软件优化,光能利用率可达90%以上。硬件电路以CH32V305FPB6芯片为主控,光源功率输出稳定,外围信号处理电路精度和采样信噪比高。结合Solidworks软件进行机械设计,将光学与电路系统集成,体积仅4.1 cm×3 cm×2 cm,可单独使用或集成于其他设备。经乙型肝炎病毒(HBV)样品测试,可快速准确判断阳性,经过高中低浓度样品检测测试10次变异系数CV值分别为0.01%、0.07%、0.62%,均小于国家标准的2%,线性实验相关系数为0.998,检测重复性和线性度好,具有良好应用前景。Aiming to address the issues of complex operation,large size,and high cost associated with tradi-tional fluorescence Polymerase Chain Reaction(PCR)detection instruments,we have developed a portable real-time fluorescence detection device.This device integrates optics,mechanics,and electronics,achieving a high level of integration.The optical system is optimized using Zemax software,resulting in an optical efficiency exceeding 90%.The hardware circuit employs the CH32V305FPB6 chip as the main controller,ensuring stable light source power output and high precision in periph-eral signal processing circuits with excellent signal-to-noise ratios.Mechanical design is conducted using SolidWorks software,integrating the optical and circuit systems into a compact unit measur-ing only 4.1 cm×3 cm×2 cm,which can function independently or be integrated into other devices.Testing with hepatitis B virus(HBV)samples demonstrates rapid and accurate positive detection.When testing samples at high,medium,and low concentrations,the coefficients of variation(CV)for ten repeated tests are 0.01%,0.07%,and 0.62%,respectively,all below the national standard of 2%,and the linear experiment correlation coefficient was 0.998,indicating excellent detection repeatability and linearity.This device holds promising application prospects.

关 键 词：便携式设备 聚合酶链式反应 荧光实时检测 光学设计 电路设计 

分 类 号：TH789[机械工程—仪器科学与技术] TH74[机械工程—精密仪器及机械]