基于网络药理学分析鱼腥草改善胰岛素抵抗的潜在靶点及作用机制  

Network Pharmacology Study of Houttuyniae Herba in Treatment of Insulin Resistance:Potential Targets and Mechanisms

作　　者：王晋星一 高杰杰 刘洋 夏杰 何露 施俊伟 邸亚男 WANG Jin-xing-yi;GAO Jie-jie;LIU Yang;XIA Jie;HE Lu;SHI Jun-wei;DI Ya-nan(Department of pharmacy,The Second Affi liated Hospital of GuiZhou Medical University,Guizhou Kaili 556000,China;Clinical Laboratory,Peking University Third Hospital,Beijing 100089,China;Department of pharmacy,Hangzhou TCM Hospital Affi liated to Zhejiang Chinese Medical University,Zhejiang Hangzhou 310007,China)

机构地区：[1]贵州医科大学第二附属医院药剂科,贵州黔东南550006 [2]北京大学第三医院检验科,北京100089 [3]浙江中医药大学附属杭州市中医院药剂科,浙江杭州310007

出　　处：《中华养生保健》2025年第6期6-11,共6页CHINESE HEALTH CARE

基　　金：贵州省中医药管理局中医药、民族医药科学技术研究项目(QZYY-2024-160);黔东南州科技计划项目[黔东南科合J字〔2023〕73号、黔东南科合基础(2023)17号]。

摘　　要：目的基于网络药理学探讨鱼腥草改善胰岛素抵抗(IR)的分子机制。方法利用中药系统药理学分析平台(TCMSP)获取鱼腥草的活性成分及作用靶点,借助Genecards数据库获取IR疾病相关靶点,利用Venny 2.1.0在线软件获得二者的交集靶点即为鱼腥草治疗IR的潜在作用靶点。利用Cystoscape 3.9.0软件及STRING数据库分别构建中药-活性成分-潜在作用靶点网络及潜在作用靶点的蛋白相互作用(PPI)网络,利用metascape数据库对鱼腥草治疗IR的潜在作用靶点进行GO功能富集和KEGG通路富集。结果共筛选出鱼腥草有效活性成分7个、84个潜在作用靶点。分析得到槲皮素、山柰酚等关键活性成分,转录因子AP-1(JUN)、细胞肿瘤抗原p53(TP53)、丝裂原活化蛋白激酶-1(MAPK1)等关键靶点。其对IR的治疗作用涉及激素应答、脂质结合等生物学过程、癌症通路、糖尿病并发症中的晚期糖基化产物及其受体信号通路、脂质和动脉粥样硬化通路等。结论鱼腥草可能主要通过槲皮素、山柰酚等活性成分调节癌症、糖尿病并发症中的晚期糖基化产物及其受体信号、脂质和动脉粥样硬化等相关信号通路发挥治疗IR的作用。Objective To explore the potential mechanism of Houttuynia herba on improving insulin resistance(IR)using network pharmacology. Methods The chemical compounds and potential targets of Houttuynia Herba were obtained byTCMSP database. Targets related to IR d were obtained by Genecards database. Meanwhile, the intersection targets of theHouttuynia herba were obtained by venny2.1.0 online. The “herbs-active components targets” and protein interaction (PPI)network of potential target were constructed by cystoscape 3.9.0 software and STRING database. The GO analysis and KEGGenrichment analysis were performed by Matascape database. Results There were 7 active components and 84 potential targets ofHouttuynia Herba for IR ameliorating. Active components with high scores were quercetin and kaempferol. The key targets weretranscription factor AP-1 (JUN), cell tumor antigen p53 (TP53), mitogen activated protein kinase-1 (MAPK1), etc. The relatedmetabolic process was related to hormone response, lipid binding and so on. The KEGG pathways were enriched in pathways incancer, AGE-RAGE signaling pathway in diabetic complications, lipid and atherosclerosis, etc. Conclusion Houttuynia Herbamay play a role in the treatment of IR mainly through quercetin and kaempferol to regulate the late glycation products and theirreceptor signals, lipids, atherosclerosis and other related signal pathways in the complications of cancer and diabetes.

关 键 词：鱼腥草 胰岛素抵抗 网络药理学 信号通路 

分 类 号：R285[医药卫生—中药学]