补肾活血方对脊髓损伤诱发骨质疏松的治疗作用及机制研究  

Study of effect and mechanism of Bushen Huoxue Formula on osteoporosis induced by spinal cord injury

作　　者：罗俊华 侯宇[2,3] 苏国义 肖志锋[4] 黄俊翰[2] 龙邀洋 高泽辉 李永津 林定坤[2,3] 詹吉恒[2,3] LUO Junhua;HOU Yu;SU Guoyi;XIAO Zhifeng;HUANG Junhan;LONG Aoyang;GAO Zehui;LI Yongjin;LIN Dingkun;ZHAN Jiheng(Second Clinical Medical College,Guangzhou University of Chinese Medicine,Guangzhou 510405,China;Department of Spinal Minimally Invasive,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510120,China;Research Team on the Prevention and Treatment of Spinal Degenerative Disease,Guangdong Provincial Academy of Chinese Medical Sciences,Guangzhou 510006,China;Spinal Department,University City Hospital,Guangdong Provincial Hospital of Chinese Medicine,Guangzhou 510006,China)

机构地区：[1]广州中医药大学第二临床医学院,广州510405 [2]广东省中医院脊柱微创科,广州510120 [3]广东省中医药科学院,脊柱退变性疾病防治研究团队,广州510006 [4]广东省中医院大学城医院脊柱科,广州510006

出　　处：《中华中医药杂志》2025年第3期1092-1097,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.82104895,No.82074451);中国博士后科学基金资助项目(No.2021M700905);广东省基础与应用基础研究基金项目(No.2019A1515110105);广州市科技计划项目(No.202201010974);广东省中医药局科研项目(No.20221179)。

摘　　要：目的:探讨补肾活血方对脊髓损伤(SCI)后骨质疏松的治疗作用,并挖掘其潜在的作用机制。方法:采用改良Allen's法构建SCI小鼠模型,BSHX-L组、BSHX-H组予灌服中药,Sham组及SCI组均予0.9%氯化钠溶液灌胃,连续干预8周。干预结束分离股骨进行检测。应用micro-CT检测骨密度、骨微细结构,三点弯曲实验检测股骨生物力学,HE染色、免疫组化、TRAP染色进行组织形态学评价,分光光度法检测过氧化物水平,RT-qPCR法检测炎症因子mRNA水平。结果:SCI小鼠在造模8周后股骨呈重度骨质疏松改变。补肾活血方能显著改善SCI小鼠股骨的骨密度、骨微细结构、骨生物力学及骨组织形态学(P<0.05,P<0.01),增加OCN、OPN阳性表达细胞并抑制TRAP阳性表达的破骨细胞(P<0.05,P<0.01)。此外,补肾活血方干预后显著降低了股骨组织中的氧化应激和炎症水平(P<0.05,P<0.01)。上述趋势在BSHX-H组中更明显。结论:补肾活血方可减轻骨组织氧化应激和炎症水平,维持骨稳态,从而减轻SCI诱发的骨质疏松。Objective:To explore the therapeutic effect of Bushen Huoxue Formula(BSHX)on osteoporosis induced by spinal cord injury(SCI),and elucidate the underlying mechanisms.Methods:SCI mouse model was established by improved Allen's method and treated according to grouping.The BSHX-L group and the BSHX-H group were given traditional Chinese medicine,and the sham group and SCI group were given normal saline intragastric administration.The intervention lasted for 8 weeks.The femurs were dissected at the end of the intervention for subsequent testing.Bone mineral density(BMD)and bone microstructure were assessed by micro-CT,femoral biomechanics was detected by 3-point bending test,and histomorphology was evaluated by HE staining,immunohistochemistry and TRAP staining.The level of oxidative stress and infiammation were detected by spectrophotometry and RT-qPCR,respectively.Results:SCI mice showed severe osteoporosis in the femurs after 8 weeks of modeling.The femoral BMD,bone microstructure,biomechanics and tissue morphology of SCI mice were significantly improved by BSHX treatment,and also increased the number of OCN-and OPN-positive cells and decreased the number of TRAP-positive osteoclastlike cells(P<0.05,P<0.01).In addition,BSHX significantly reduced oxidative stress and inflammation in the femurs(P<0.05,P<0.01).These trends were more pronounced in theBSHX-H group(P<0.05).Conclusion:BSHX can alleviate SCI-induced osteoporosis by reducing oxidative stress and inflammation and maintaining bone homeostasis.

关 键 词：脊髓损伤 骨质疏松 补肾活血方 氧化应激 炎症 骨稳态 

分 类 号：R285.5[医药卫生—中药学]