许氏平鲉和褐菖鲉全基因组微卫星分布规律及其比较分析  

Distribution and comparative analysis of whole genome microsatellite in Sebastes schlegelii and Sebastiscus marmoratus

作　　者：瞿才惠 瞿印权 贺艳[2] 赵祥 高天翔 QU Caihui;QU Yinquan;HE Yan;ZHAO Xiang;GAO Tianxiang(School of Fishery,Zhejiang Ocean University,Zhoushan 316022,China;College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China;Fisheries College,Ocean University of China,Qingdao 266003,China)

机构地区：[1]浙江海洋大学水产学院,浙江舟山316022 [2]中国海洋大学海洋生命学院,山东青岛266003 [3]中国海洋大学水产学院,山东青岛266003

出　　处：《水产学报》2025年第5期29-40,共12页Journal of Fisheries of China

基　　金：国家自然科学基金(32072966)。

摘　　要：【目的】探究许氏平鲉和褐菖鲉全基因组中微卫星的分布规律。【方法】使用MISA软件对许氏平鲉和褐菖鲉两种鱼类全基因组中完整型微卫星序列进行搜索和统计,通过Excel软件制作图表,对微卫星统计结果进行比较分析。【结果】许氏平鲉基因组序列总长度为848024649 bp,序列总数为741条,其中有635条序列含有534272个微卫星位点,微卫星序列总长度为14151681 bp,占基因组总长度的1.67%,平均每1587.25 bp含有1个微卫星位点。不同重复类型微卫星表现为二碱基(46.37%)>单碱基(30.97%)>三碱基(15.42%)>四碱基(5.7%)>五碱基(1.19%)>六碱基(0.35%)。褐菖鲉基因组序列总长度为795691141 bp,序列总数为90条,其中有77条序列含有571370个微卫星位点,微卫星序列总长度为15854826 bp,占基因组总长度的1.99%,平均每1392.60 bp含有1个微卫星位点。不同重复类型微卫星表现为二碱基(47.63%)>单碱基(32.90%)>三碱基(13.63%)>四碱基(4.26%)>五碱基(1.04%)>六碱基(0.53%)。【结论】许氏平鲉和褐菖鲉的不同重复类型微卫星表现相同。随着微卫星序列扩增长度的增加,两种鱼类核苷酸出现的频次整体呈逐渐下降的趋势。本研究对许氏平鲉和褐菖鲉全基因组微卫星分布规律进行了比较分析,为今后两种鱼类种群遗传结构和遗传多样性等研究奠定了基础。To elucidate the distribution of microsatellites in the genomes of Sebastes schlegelii and Sebastiscus marmoratus,the MISA software was employed to search for and compare microsatellite sequences across both genomes.The results indicated that the total length of the S.schlegelii genome sequence was 848024649 bp,comprising 741 sequences,of which 635 contained 534272 microsatellite loci.The total length of microsatellite sequence was 14151681 bp,with an average of one microsatellite locus per 1587.25 bp,representing 1.67%of the genome.Of the sequences containing microsatellite loci,584(91.97%)contained more than one locus,while 51(8.03%)contained a single locus.The ratios of different repeat types of microsatellites,ranked by size,were as follows:di-nucleotide(46.37%)>mono-nucleotide(30.97%)>tri-nucleotide(15.42%)>tetra-nucleotide(5.7%)>penta-nucleotide(1.19%)>hexa-nucleotide(0.35%).For S.marmoratus,the total genome sequence length was 795691141 bp,with a total of 90 sequences.Among these,77 sequences contained 571370 microsatellite loci.The total length of microsatellite sequences was 15854826 bp,with an average of one microsatellite locus per 1392.60 bp,accounting for 1.99%of the genome.Of the sequences containing microsatellite locus,74(96.10%)contained more than one locus,while 3(3.70%)sequences contained a single locus.The ratios of different repeat types of microsatellites,ranked by size,were di-nucleotide(47.63%)>mono-nucleotide(32.90%)>tri-nucleotide(13.63%)>tetra-nucleotide(4.26%)>penta-nucleotide(1.04%)>hexa-nucleotide(0.53%).Our findings demonstrate that the microsatellites of different repeat types in S.schlegelii and S.marmoratus exhibit similar characteristics.Additionally,as the amplification length of microsatellite sequences increased,the frequency of nucleotides in both fish species showed a gradual downward trend.This study,which compared the distribution of microsatellites in the genomes of S.schlegelii and S.marmoratus,provided a foundation for future research on microsatellite marker screening

关 键 词：许氏平鲉 褐菖鲉 全基因组 微卫星 特征分析 

分 类 号：S917.4[农业科学—水产科学]