基于UHPLC-Q-Exactive Orbitrap MS/MS联用技术的草乌及炮制品化学成分快速鉴定  

Rapid identification of chemical components in Aconiti Kusnezoffii Radix and processed products based on UHPLC-Q-Exactive Orbitrap MS/MS combined technology

作　　者：布仁 高晨阳 马广平 莲花 白文明 BU Ren;GAO Chen-yang;MA Guang-ping;LIAN Hua;BAI Wen-ming(College of Pharmacy,Hohhot 010110,China;College of Traditional Mongolian Medicine,Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学药学院,内蒙古呼和浩特010110 [2]内蒙古医科大学蒙医药学院,内蒙古呼和浩特010110

出　　处：《化学研究与应用》2025年第4期854-863,共10页Chemical Research and Application

基　　金：内蒙古医科大学面上项目(YKD2021MS022)资助;内蒙古医科大学教学创新团队项目(NYCXTD202404)资助;内蒙古自治区蒙医药标准化项目(2023-[MB027])资助。

摘　　要：本研究采用超高效液相色谱-四极杆/静电场轨道高分辨质谱(UHPLC-Q-Exactive Orbitrap MS/MS)对草乌及其炮制品中的化学成分进行了快速鉴定。实验使用Acclaim 120 C_(18)色谱柱(2.1 mm×100 mm,3μm),以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,柱温为30℃,流速为0.3 mL·min^(-1),进样量为10μL。同时,基于电喷雾离子源(ESI),于正、负离子扫描模式下采集样品数据。通过数据解析,共鉴定得232个化合物,其中包括生物碱类105个(主要为C_(18)、C_(19)和C_(20)型二萜生物碱)、有机酸类54个、氨基酸及其衍生物33个、糖类11个、核苷酸9个和其他类20个。本研究结果能够为草乌及其炮制品药效物质基础研究提供参考,也可作为草乌药材深入研究的基础。This study used ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitrap high-resolution mass spectrometry(UHPLC-Q-Exactive Orbitrap MS/MS) to rapidly identify the chemical components in Aconiti Kusnezoffii Radix and its processed products.The experiment used an Acclaim 120 C_(18) chromatographic column(2.1 mm×100 mm,3 μm) with acetonitrile 0.1%formic acid aqueous solution as the mobile phase for gradient elution.The column temperature was 30 ℃,the flow rate was 0.3 mL·min^(-1),and the injection volume was 10 μL.At the same time,based on the electric spray ion source(ESI),sample data are collected in the positive and negative ion scanning modes.Through data analysis,a total of 232 compounds were identified,including 105 alkaloids(mainly C_(18),C_(19),and C_(20) diterpenoid alkaloids),54 organic acids,33 amino acids and their derivatives,11sugars,9 nucleotides,and 20 other classes.The results of this study can provide reference for the research on the pharmacological substance basis of Aconiti Kusnezoffii Radix and its processed products,and can also serve as the basis for in-depth research on Aconiti Kusnezoffii Radix medicinal materials.

关 键 词：草乌 高分辨质谱法 化学成分 快速鉴定 数据解析 

分 类 号：O657.6[理学—分析化学]