负载MXene纳米片的冻融水凝胶的制备及防治术后胰瘘的研究  

Preparation of Freeze-Thaw Hydrogels Loaded with MXene Nanosheets and Their Prevention and Treatment of Postoperative Pancreatic Fistula

作　　者：刘皓珩 郭倩如 田锦[1] 门杰 李可洲 LIU Haoheng;GUO Qianru;TIAN Jin;MEN Jie;LI Kezhou(College of Medicine,Southwest Jiaotong University,Chengdu 610031,China;School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;Department of Pancreatic Surgery,West China Hospital of Sichuan University,Chengdu 610041,China)

机构地区：[1]西南交通大学医学院,成都610031 [2]西南交通大学材料科学与工程学院,成都610031 [3]四川大学华西医院胰腺外科,成都610041

出　　处：《材料导报》2025年第9期220-228,共9页Materials Reports

基　　金：四川省重点研发项目(2023YFS0182)。

摘　　要：术后胰瘘(Postoperative pancreatic fistula,POPF)是胰腺切除术后最常见和最严重的并发症之一,会导致住院时间延长、医疗保健成本增加以及引发潜在的危及生命的并发症。目前在临床上用于防治术后胰瘘的胰管支架、密封胶和聚乙醇酸毡等材料虽然具有一定的作用,但是仍面临感染及愈合速率慢等问题。受到创面水凝胶敷料的启发,制备一种负载MXene纳米片的PVA-CS-MXene冻融水凝胶,其具有良好的力学性能、吸水溶胀行为、生物降解性能和细胞相容性;在血液相容性实验中,水凝胶材料的溶血率低于5%,证明其具有良好的血液相容性;抗菌实验证明PVA-CS水凝胶具有一定的抗菌性能,并且通过MXene的载入显著地提升了水凝胶的抗菌性能。建立大鼠术后胰瘘模型,通过残余胰腺组织创面愈合实验,证明PVA-CS-MXene冻融水凝胶能抑制创面炎症反应,促进残余胰腺组织创面愈合,从而有效防治术后胰瘘。实验结果表明,PVA-CS-MXene冻融水凝胶有望成为胰腺切除术后胰瘘防治的一种有前景的材料。Postoperative pancreatic fistula(POPF)is one of the most common and serious complications after pancreatectomy,resulting in prolonged hospital stays,increased healthcare costs and potentially life-threatening complications.At present,pancreatic duct stents,sealant and polyglycolic acid felt used in clinical prevention and treatment of postoperative pancreatic fistula have certain effects,but they still face problems such as infection and slow healing rate.Inspired by the wound hydrogel dressing,in this study,we prepared PVA-CS-MXene freeze-thaw hydrogel loaded with MXene nanosheets,which has good mechanical properties,swelling behavior,biodegradability and cytocompatibility.In hemocompatibility experiments,the hemolysis rate of hydrogel materials was less than 5%,which proved that PVA-CS hydrogels had good hemocompatibility.Antimicrobial experiments showed that PVA-CS hydrogels had certain antibacterial properties,and the antibacterial properties of the hydrogels were significantly improved by MXene loading.A rat model of postoperative pancreatic fistula was established and wound healing experiment of the residual pancreatic tissue proved that PVA-CS-MXene freeze-thaw hydrogel could inhibit the inflammatory response of the wound and promote the wound healing of the residual pancreatic tissue,so as to effectively prevent and treat postoperative pancreatic fistula.The experimental results show that PVA-CS-MXene freeze-thaw hydrogel is expected to be a promising material for the prevention and treatment of pancreatic fistula after pancreatectomy.

关 键 词：冻融水凝胶 MXene 抗炎 抗菌 创面愈合 术后胰瘘 

分 类 号：R318.08[医药卫生—生物医学工程]