槲皮素对宫颈炎大鼠阴道微生态、局部细胞因子及PI3K、Akt蛋白的影响  

Effects of quercetin on vaginal microecology,local cytokines,PI3K and Akt in cervicitis rats

作　　者：许涵 王静 孙丽妍 康聪[1] XU Han;WANG Jing;SUN Liyan;KANG Cong(Department of Gynecology,Harrison International Peace Hospital,Hengshui,Hebei 053000,China)

机构地区：[1]哈励逊国际和平医院妇科,河北衡水053000

出　　处：《检验医学与临床》2025年第8期1061-1066,1076,共7页Laboratory Medicine and Clinic

基　　金：河北省卫生健康委员会医学科学研究基金项目(20211763)。

摘　　要：目的 探讨槲皮素对宫颈炎大鼠阴道微生态、局部细胞因子及磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(Akt)蛋白的作用机制,为宫颈炎相关用药提供参考依据。方法 60只大鼠按照随机数字表法分为正常组、模型组、西药组、槲皮素低剂量、槲皮素中剂量及槲皮素高剂量组,每组10只。除正常组外,其余大鼠均建立宫颈炎大鼠模型,槲皮素低、中、高剂量组大鼠阴道分别注入浓度为35、55、80μmol/L的槲皮素,西药组大鼠注入阴道0.081 g/kg的抗宫颈炎片研磨冲剂,正常组、模型组大鼠阴道注入等量的生理盐水。采用全自动阴道微生态检测仪检测阴道微生态的pH值、分泌型免疫球蛋白A(SIgA)及乳酸杆菌,采用全自动血细胞计数分析法检测白细胞、中性粒细胞计数,酶联免疫吸附试验检测血清白细胞介素(IL)-1β、IL-6、IL-8水平,苏木精-伊红(HE)染色法观察大鼠宫颈组织情况,采用免疫印迹法检测PI3K、Akt蛋白表达水平。结果 各组pH值、乳酸杆菌异常比例、SIgA水平、白细胞计数、中性粒细胞计数,IL-1β、IL-6、IL-8水平及PI3K、Akt蛋白表达水平比较结果显示,正常组<槲皮素高剂量组<槲皮素中剂量组<槲皮素低剂量组<模型组,任意2组间比较,差异均有统计学意义(P<0.05)。西药组pH值、乳酸杆菌异常比例、SIgA水平、白细胞计数、中性粒细胞计数及血清IL-1β、IL-6、IL-8水平,以及PI3K、Akt蛋白表达水平高于正常组,低于槲皮素中剂量组、槲皮素低剂量组、模型组,差异均有统计学意义(P<0.05)。治疗后,槲皮素高剂量组、槲皮素中剂量组、槲皮素低剂量组、西药组宫颈评分高于治疗前,差异均有统计学意义(P<0.05)。治疗后,各组宫颈评分比较结果显示,模型组<槲皮素低剂量组<槲皮素中剂量组<槲皮素高剂量组<正常组,任意2组间比较,差异均有统计学意义(P<0.05)。西药组宫颈评分高于模型组、槲皮素低剂�Objective To explore the mechanism of quercetin on vaginal microecology,local cytokines,phosphoinositide 3-kinase(PI3K)and protein kinase B(AKT)proteins in cervicitis rats,and to provide a reference for the related medication of Cervicitis.Methods According to random number table method,60 rats were divided into normal group,model group,western medicine group,quercetin low-dose,quercetin medium-dose and quercetin high-dose groups,with 10 rats in each group.Except the normal group,the other rats were established with cervical inflammation model.The quercetin low-dose,medium-dose,high-dose groups rats were injected with quercetin with vaginal concentration of 35,55 and 80μmol/L respectively,and the western medicine group rats were injected with anti-cervictis tablet grinding powder of 0.081 g/kg.Rats in normal group and model group were injected into the vagina with an equal amount of normal saline.The pH value,secreted immunoglobulin A(SIgA)and lactobacillus of vaginal microecology were detected by automatic vaginal microecology detector.The white blood cells neutrophils were classified by automatic blood count analysis,and the levels of serum interleukin(IL)-1β,IL-6 and IL-8 were detected by enzyme-linked immunosorbent assay.The cervical tissue of rats was observed by hematoxylin-eosin staining.The expressions of PI3K and Akt were detected by western blot.Results The comparison of pH value,abnormal proportion of lactobacillus,SIgA level,white blood cell count,neutrophil count,serum IL-1β,IL-6,IL-8 levels,and PI3K and Akt expression levels among groups showed that normal group<quercetin high-dose group<quercetin medium-dose group<quercetin low-dose group<model group,and comparison between any two groups,and the differences were statistically significant(P<0.05).The pH value,abnormal proportion of lactobacillus,SIgA level,white blood cell count,neutrophil count,serum IL-1β,IL-6 and IL-8 levels and the expression levels of PI3K and Akt protein in western medicine group were higher than those in normal group,but

关 键 词：槲皮素 宫颈炎 阴道微生态 细胞因子 磷脂酰肌醇3激酶 蛋白激酶B 

分 类 号：R711.74[医药卫生—妇产科学] R33[医药卫生—临床医学]