隔药灸脐法对失眠大鼠的治疗作用及机制  

Therapeutic Effect and Mechanism of Herb-partitioned Moxibustion at the Navel in Insomnia Rats

作　　者：叶小娜[1] 郑雅峰 孙超[1] 宋洪玉[1] 赵荧荧 王文秀 刘露露 林秀群 赵立杰[1] 马玉侠[2] 韩兴军[1] YE Xiaona;ZHENG Yafeng;SUN Chao;SONG Hongyu;ZHAO Yingying;WANG Wenxiu;LIU Lulu;LIN Xiuqun;ZHAO Lijie;MA Yuxia;HAN Xingjun(The Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Ji′nan 250001,China;Shandong University of Traditional Chinese Medicine,Ji′nan 250355,China)

机构地区：[1]山东中医药大学第二附属医院,济南250001 [2]山东中医药大学,济南250355

出　　处：《世界中医药》2025年第2期252-258,共7页World Chinese Medicine

基　　金：国家重点研发计划项目(2022YFC3500403)。

摘　　要：目的:研究隔药灸脐法对失眠大鼠下丘脑组织形态及外周血中皮质酮(CORT)、前列腺素D2(PGD2)、白细胞介素-1β(IL-1β)的影响,探讨其改善失眠的作用机制。方法:将27只雄性斯泼累格·多雷(SD)大鼠按随机数字表分为空白组、模型组和观察组,每组9只。模型组和观察组均采用氯苯丙氨酸(PCPA)腹腔注射改良法制造大鼠模型。模型制备方法:配置制PCPA弱碱性生理盐水溶液,按0.5 g/kg进行腹腔注射,连续注射2 d。模型制备完成后,空白组仅腹腔注射同等体积生理盐水,不给予其他任何干预。模型组仅固定大鼠四肢,观察组给予隔药灸脐法进行干预,每隔2天治疗1次,共治疗10次。干预结束后,将大鼠腹腔注射戊巴比妥钠麻醉后取腹主动脉血;心脏灌注后取脑分离下丘脑。观察各组大鼠一般情况,戊巴比妥钠翻正实验评价睡眠情况,苏木精-伊红(HE)染色观察下丘脑组织形态变化,酶联免疫吸附试验(ELISA)检测大鼠血清中血激素(CORT、PGD2)及炎症介质(IL-1β)。结果:与模型组比较,观察组大鼠一般情况均有不同程度改善。干预前,与空白组比较,模型组、观察组大鼠体质量低(P<0.05)。干预后,与空白组比较,模型组大鼠体质量低(P<0.05);与模型组比较,观察组大鼠体质量高(P<0.05)。与本组干预前比较,模型组大鼠体质量降低(P<0.05),空白组、观察组大鼠体质量升高(P<0.05)。造模后与空白组比较,模型组与观察组大鼠睡眠潜伏期长(P<0.05),睡眠时间短(P<0.05);干预后与空白组比较,模型组大鼠睡眠潜伏期长(P<0.05),睡眠时间短(P<0.05),与模型组比较,观察组大鼠睡眠潜伏期短(P<0.05),睡眠时间长(P<0.05);模型组中与造模前比较,造模后与干预后睡眠潜伏期长(P<0.05),睡眠时间短(P<0.05);观察组中与造模前比较,造模后睡眠潜伏期长(P<0.05),睡眠时间短(P<0.05),与造模后比较,大鼠睡眠潜伏期短(P<0.05),睡眠时间长(P<0.05)。HE染Objective:To investigate the effects of herb-partitioned moxibustion at the navel on the hypothalamic tissue morphology and peripheral blood levels of corticosterone(CORT),prostaglandin D2(PGD2),and interleukin-1β(IL-1β)in rats with insomnia,and to explore its mechanism in improving insomnia.Methods:Twenty-seven male Sprague-Dawley(SD)rats were randomly divided into three groups according to a random number table:blank group,model group,and treatment group,with 9 rats in each group.The insomnia model was induced in the model and treatment groups by intraperitoneal injection of P-chlorophenylalanine(PCPA)using a modified method.Model establishment method:The PCPA solution was prepared in weakly alkaline physiological saline and administered by intraperitoneal injection at 0.5 g/kg for 2 consecutive days.After model establishment,the blank group was treated with equal volume of physiological saline and no further intervention.The model group received fixation of four limbs without additional intervention,while the treatment group received herb-partitioned moxibustion at the navel once every 2 days for a total of 10 treatments.After the intervention,rats were anesthetized by intraperitoneal injection with pentobarbital sodium,and blood was collected from the abdominal aorta.The hypothalamus was isolated after heart perfusion.The general condition of the rats was observed,and sleep was assessed by the pentobarbital sodium sleep test.Hematoxylin-eosin(HE)staining was used to observe the morphology of the hypothalamus,and enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of CORT,PGD2,and inflammatory mediator IL-1βin serum.Results:Compared to the model group,the general condition of the treatment group improved to varying degrees.Before the intervention,the model and treatment groups had lower body weight than the blank group(P<0.05).After the intervention,the body weight of the model group was lower than that in the blank group(P<0.05),and the treatment group was higher than the model group(P<0.

关 键 词：失眠 隔药灸脐 对氯苯丙氨酸 下丘脑 组织形态 炎症介质 神经递质 

分 类 号：R245[医药卫生—针灸推拿学]