基于细胞自噬探讨玉屏风散对变应性鼻炎大鼠炎症因子影响的实验研究  

Experimental Study on the Effect of Yupingfeng San on Inflammatory Factors in Allergic Rhinitis Rats Based on Cellular Autophagy

作　　者：米光熙 王仲夏 王天秀 刘婉婷 王杰 MI Guangxi;WANG Zhongxia;WANG Tianxiu;LIU Wanting;WANG Jie(Department of Otolaryngology,Hongqi Hospital Affiliated to Mudanjiang Medical University,Mudanjiang,Heilongjiang 157000,China)

机构地区：[1]牡丹江医科大学附属红旗医院耳鼻喉头颈外科,黑龙江牡丹江157000

出　　处：《中国医药指南》2025年第12期10-14,共5页Guide of China Medicine

基　　金：黑龙江省省属高等学校基本科研业务费科研项目(2023-KYYWFMY-0035)。

摘　　要：目的 观察玉屏风散对变应性鼻炎(AR)模型大鼠细胞自噬及炎症因子的影响,探索玉屏风散改善AR的潜在作用机制。方法 将Sprague Dawley大鼠随机分为正常对照组、AR模型组、玉屏风散组(24 mg/kg)、酮替芬组(阳性对照,0.9 mg/kg)、玉屏风散(24 mg/kg)+雷帕霉素(自噬诱导剂,1 mg/kg)组,每组10只。正常对照组给予200 mg/kg生理盐水,其余各组以卵清蛋白和氢氧化铝建立AR模型。记录AR大鼠鼻炎行为学症状并量化评分;采用免疫印迹和免疫荧光检测大鼠鼻黏膜自噬效应蛋白Beclin1的表达;ELISA检测大鼠鼻腔灌洗液和血清中干扰素γ(INF-γ)、白细胞介素-5(IL-5)、白细胞介素-13(IL-13)、免疫球蛋白E(IgE)及白细胞介素-4(IL-4)水平。结果 与正常对照组比较,AR模型组大鼠鼻腔灌洗液和血清中IL-5、IL-13、IgE和IL-4水平升高(P<0.01),INF-γ水平降低(P<0.01);与AR模型组比较,玉屏风散组和酮替芬组大鼠鼻腔灌洗液和血清中IL-5、IL-13、IgE和IL-4水平减少(P<0.01),INF-γ水平升高(P<0.01);Western Blot结果显示,与正常对照组比计较,AR模型组自噬相关蛋白Beclin1表达显著增加(P<0.05);与AR模型组比较,玉屏风散组和酮替芬组Beclin1蛋白表达显著减少(P<0.01);免疫荧光结果显示,与玉屏风散组比较,玉屏风散组+雷帕霉素组Beclin1蛋白表达显著增加,大鼠鼻腔灌洗液和血清中IL-5、IL-13、IgE和IL-4水平显著增加(P<0.01),INF-γ水平明显减少(P<0.01),大鼠鼻炎行为学评分显著增加(P<0.01)。结论 玉屏风散对AR具有良好疗效,其潜在作用机制与细胞自噬密切相关。Objective To observe the effects of Yupingfeng San on autophagy and inflammatory factors in allergic rhinitis(AR)model rats,and explore the potential mechanism of Yupingfeng San in improving AR.Methods Sprague Dawley rats were randomly divided into a normal control group,an AR model group,a Yupingfeng San group(24 mg/kg),a ketotifen group(positive control,0.9 mg/kg),and a Yupingfeng San(24 mg/kg)+rapamycin(autophagy inducer,1 mg/kg)group,with 10 rats in each group.The normal control group was given 200 mg/kg physiological saline,while the other groups established AR models using ovalbumin and aluminum hydroxide.Record the behavioral symptoms of rhinitis in AR rats and quantify the scores.Immunoblotting and immunofluorescence were used to detect the expression of Beclin1,an autophagy related protein,in rat nasal mucosa.ELISA was used to detect the levels of interferon-γ(INF-γ),interleukin-5(IL-5),interleukin-13(IL-13),immunoglobulin E(IgE)and interleukin-4(IL-4)in nasal lavage fluid and serum of rats.Results Compared with the normal control group,the levels of IL-5,IL-13,IgE,and IL-4 in the nasal lavage fluid and serum of the AR model group rats were significantly increased(P<0.01),while the level of INF-γwas significantly decreased(P<0.01).Compared with the AR model group,the levels of IL-5,IL-13,IgE,and IL-4 in nasal lavage fluid and serum of rats in the Yupingfeng San group and Ketotifen group were significantly reduced(P<0.01),while the levels of INF-γwere significantly increased(P<0.01).Western blot results showed that compared with the normal control group,the AR model group showed a significant increase in the expression of autophagy related protein Beclin1(P<0.05).Compared with the AR model group,the expression of Beclin1 protein was significantly reduced in the Yupingfeng San group and Ketotifen group(P<0.01).The immunofluorescence results showed that compared with the Yupingfeng San group,the Yupingfeng San+rapamycin group showed a significant increase in Beclin1 protein expression,a significant incr

关 键 词：变应性鼻炎 玉屏风散 细胞自噬 炎症因子 机制 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]