清热安胎膏改善大鼠妊娠期龈炎及对SOD、CAT活性的影响  

Effect of Qingre Antai Paste on the Improvement of Gingivitis and the Activity of SOD and CAT in Rats During Pregnancy

作　　者：陆静宇 陈世娟[1] 王楚睿 康静 LU Jingyu;CHEN Shijuan;WANG Churui;KANG Jing(Department of Stomatology,the First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410007,Hunan,China)

机构地区：[1]湖南中医药大学第一附属医院口腔科,湖南长沙410007

出　　处：《医学信息》2025年第8期65-69,共5页Journal of Medical Information

基　　金：湖南省自然科学基金项目(编号:211142196052)。

摘　　要：目的 观察中药清热安胎膏对大鼠妊娠期龈炎的疗效、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响。方法 从北京维通利华公司购入24只9周龄SPF级别的SD雌鼠,12只雄鼠,采用简单随机分组的方法将雌鼠均等随机分配至四个实验组别:空白对照组、模型组、阳性药物对照组及中药组,每组包含6只大鼠。适应性喂养后构建实验性龈炎,7 d后合笼受孕,受孕3 d后予以分组处理。并拍摄记录各组大鼠造模后和分组处理后牙龈状态。空白对照组:不做任何处理,常规受孕;模型组:构建实验性龈炎后受孕;阳性药物对照组:构建实验性龈炎,受孕后龈沟处予以碘甘油;中药组:构建实验性龈炎,受孕后龈沟处予以填塞清热安胎膏。分组处理后,切取牙龈组织,酶联免疫吸附试验(ELISA)测定实验大鼠牙龈组织中SOD和CAT的活性水平。结果 大鼠造模后,双下门齿牙龈出现明显充血,红肿等情况。妊娠期各组大鼠处理后牙龈红肿程度:模型组>中药组≥阳性药物对照组>空白对照组。与空白对照组相比,模型组、阳性药物对照组、中药组SOD、CAT活性降低(P<0.05);中药组SOD、CAT活性高于模型组(P<0.05)。结论 清热安胎膏可以有效提高大鼠妊娠期龈炎中SOD、CAT活性,改善氧化应激,减轻牙龈组织红肿及损伤,有望成为治疗妊娠期龈炎的新方法。Objective To observe the effect of Qingre Antai paste on the activity of superoxide dismutase(SOD) and catalase(CAT) in rats with gingivitis during pregnancy.Methods A total of 24 9-week-old SPF SD female rats and 12 male rats were purchased from Beijing Charles River Company.Female rats were randomly divided into four experimental groups:blank control group,model group,positive drug control group and traditional Chinese medicine group,with 6 rats in each group.After adaptive feeding,experimental gingivitis was constructed.After 7 days,they were conceived in a cage,and after 3 days of conception,they were grouped.The gingival state of rats in each group after modeling and group treatment was recorded.Blank control group:without any treatment,conventional conception;model group:pregnancy after experimental gingivitis was constructed;positive drug control group:experimental gingivitis was constructed,and iodine glycerin was given at the gingival sulcus after conception;traditional Chinese medicine group:experimental gingivitis was constructed,and Qingre Antai paste was filled in the gingival sulcus after conception.After grouping treatment,the gingival tissue was cut,and the activity levels of SOD and CAT in the gingival tissue of the experimental rats were determined by enzyme-linked immunosorbent assay(ELISA).Results After modeling,the gums of the lower incisors of the rats showed obvious congestion,redness and swelling.The degree of gingival swelling after treatment in each group of rats during pregnancy:model group >traditional Chinese medicine group≥positive drug control group>blank control group.Compared with the blank control group,the activity of SOD and CAT in the model group,the positive drug control group and the traditional Chinese medicine group decreased( P<0.05).The activities of SOD and CAT in the traditional Chinese medicine group were higher than those in the model group(P<0.05).Conclusion Qingre Antai paste can effectively improve the activity of SOD and CAT in rats with gingivitis during pregn

关 键 词：妊娠期龈炎 清热安胎膏 氧化应激 

分 类 号：R276.8[医药卫生—中医五官科学]