利水保肝巴布剂对肝腹水的药效评价  

Pharmacodynamic Evaluation of Lishui Baogan Cataplasm on Hepatic Ascites

作　　者：丁艳霞[1] 王佳 司玉环 杜雅情 余聪睿 刘晨 魏倩囡 DING Yanxia;WANG Jia;SI Yuhuan;DU Yaqing;YU Congrui;LIU Chen;WEI Qiannan(School of Pharmacy,Henan University,Kaifeng 475000,Henan,China;Medical Research Center,the First Affiliated Hospital of Henan University,Kaifeng 475000,Henan,China)

机构地区：[1]河南大学药学院,河南开封475000 [2]河南大学第一附属医院医学研究中心,河南开封475000

出　　处：《医学信息》2025年第8期118-122,共5页Journal of Medical Information

基　　金：国家级大学生创新创业训练计划(编号:202310475017);河南大学大学生创新创业训练计划(编号:校发[2022]137号)。

摘　　要：目的 考察利水保肝巴布剂治疗肝腹水的疗效。方法 采用H22瘤细胞造小鼠肝腹水模型。造模1天后,空白组、模型组给予空白基质贴,阳性药组灌胃呋塞米混悬液[6 mg/(kg·d)],巴布剂低、中、高剂量组分别给含生药剂量为0.15 g/(cm^(2)·d),0.30 g/(cm^(2)·d),0.60 g/(cm^(2)·d),给药面积4 cm^(2),每天1次,连续给药10 d。称量小鼠的体质量,测量腹围,统计小鼠死亡数,记录小鼠尿量和腹水量;ELISA检测小鼠血清白蛋白(ALB)、总蛋白(TP)、谷草转氨酶(AST)、谷丙转氨酶(ALT)含量,HE染色观察小鼠肝组织的病变情况。结果 空白组小鼠健康状态良好,体重、腹围正常,无死亡现象。与空白组相比,模型组小鼠体重、腹围增加(P<0.05);与空白组比较,模型组小鼠死亡率升高,达54.54%;与空白组相比,模型组小鼠平均尿量减少(P<0.01);与模型组相比,阳性药组及巴布剂低、中、高剂量组尿量增多,巴布剂各剂量组小鼠血清AST、ALT含量降低,TP含量增加(P<0.05);HE染色肝脏切片显示,与模型组比较,巴布剂各剂量组肝损伤均有不同程度的减轻。结论 利水保肝巴布剂对肝腹水,具有明显的消腹水和保肝作用。Objective To investigate the effect of Lishui Baogan cataplasm for the treatment of hepatic ascites.Methods The mouse model of hepatic ascites was established by H22 tumor cells.One day after modeling,the blank group and the model group were given blank matrix paste,the positive drug group was given furosemide suspension [6 mg/(kg·d)],and the low,medium and high dose groups of cataplasm were given crude drug doses of0.15 g/(cm^(2)·d),0.30 g/(cm^(2)·d),0.60 g/(cm^(2)·d),respectively,the administration area was 4 cm^(2),once a day for 10 days.The body weight of mice was weighed,the abdominal circumference was measured,the number of deaths in mice was counted,and the urine volume and ascites volume of mice were recorded.The contents of serum albumin(ALB),total protein(TP),aspartate aminotransferase(AST) and alanine aminotransferase(ALT) in mice were detected by ELISA,and the pathological changes of liver tissue in mice were observed by HE staining.Results The mice in the black group were in good health with normal weight and abdominal circumference,and no signs of death.Compared with the black group,the weight and abdominal circumference of mice in the low,medium and high dose groups of cataplasm significantly increased( P<0.05).Compared with the blank group,the mortality rate of the model group was significantly increased,reaching 54.54%.Compared with the blank group,the average urine volume of mice in the model group decreased(P<0.01).Compared with the model group,the urine volume of the positive drug group and the low,medium and high dose groups of cataplasm increased,and the ascites of the positive drug group and the low,medium and high dose groups of cataplasm decreased to varying degrees,among which the ascites of the medium dose group decreased significantly(P<0.05).The serum AST and ALT levels of mice in each dose group of cataplasm were reduced,while the TP level was increased(P<0.05).HE staining sections of liver tissue showed that compared with the model group,liver injury in each dose group of catapl

关 键 词：巴布剂 肝腹水 药效评价 

分 类 号：R285.5[医药卫生—中药学]