外周血中性粒细胞特殊亚群中CD62L水平变化作为脓毒症监测指标的研究  

CD62L expression on special subpopulations of neutrophils in peripheral blood as an indicator for sepsis monitoring

作　　者：成亚瑾 陈思邈 茹铉雯 陈丹蕾 邵青意 叶青[1] Cheng Yajin;Chen Simiao;Ru Xuanwen;Chen Danlei;Shao Qingyi;Ye Qing(Department of Clinical Laboratory,Children′s Hospital,Zhejiang University School of Medicine,Hangzhou 310051,China;School of Medical Technology and Information Engineering,Zhejiang Chinese Medical University,Hangzhou 310053,China)

机构地区：[1]浙江大学医学院附属儿童医院实验检验中心,杭州310051 [2]浙江中医药大学医学技术与信息工程学院,杭州310053

出　　处：《中华微生物学和免疫学杂志》2025年第3期239-247,共9页Chinese Journal of Microbiology and Immunology

摘　　要：目的探索脓毒症外周血中性粒细胞的异质性,为脓毒症诊断提供帮助。方法采用完全随机设计将24只雄性C57BL/6小鼠分为对照组和脓毒症组,每组12只,对照组于小鼠尾静脉注射生理盐水100μl,脓毒症组于小鼠尾静脉注射等量3.33麦氏浊度单位的大肠埃希菌溶液建立小鼠脓毒症模型。收集小鼠外周血,质谱流式技术检测表达不同表面标志物的中性粒细胞比例。对特异性高表达CD62L的中性粒细胞亚群进行GO和KEGG分析,利用公共数据集进行分析验证,并对CD62L进行互作网络分析,探索中性粒细胞异质性提示脓毒症的可能性。结果脓毒症组与对照组外周血样本间标记表达具有明显的差异性,脓毒症组中存在CD62L+中性粒细胞亚群。GO和KEGG结果显示CD62L+中性粒细胞亚群与多个细胞黏附、迁移、表面受体激活和免疫调节等生物学过程和信号传导途径相关。临床结果验证了中性粒细胞CD62L表达水平与脓毒症严重程度和预后相关。脓毒症组外周血中性粒细胞表面表达CD62L的亚群数量明显高于对照组,但脓毒症组单细胞CD62L的表达量却显著低于对照组(P<0.01)。互作网络分析显示CD62L与P-选择素、P-选择素配体、E-选择素、血管细胞黏附分子1等重要蛋白质有强烈互作关系。结论脓毒症小鼠与对照小鼠中性粒细胞表面标志物存在异质性,可为脓毒症诊断提供帮助。ObjectiveTo investigate the heterogeneity of peripheral blood neutrophils in sepsis and provide reference for the diagnosis of sepsis.MethodsTwenty-four male C57BL/6 mice were divided into two groups,control and sepsis groups,with 12 mice in each group using a completely randomized design.The mice in the control group were injected with 100μl saline through the tail vein,while the mice in the sepsis group were injected with an equal amount of Escherichia coli solution(3.33 McFarland turbidity standards)through the tail vein to establish the sepsis model.Peripheral blood samples were collected,and the proportions of neutrophils expressing different surface markers were detected using mass cytometry.GO and KEGG analyses were performed on neutrophil subsets with high CD62L expression,and public datasets were used for verification.The protein-protein interaction networks of CD62L were investigated to assess the value of neutrophil heterogeneity in the diagnosis of sepsis.ResultsThere were significant differences in the expression of markers in peripheral blood samples between the sepsis group and the control group.CD62L+neutrophil subsets were found in mice with sepsis.GO and KEGG analyses showed that CD62L+neutrophil subsets were associated with multiple biological processes and signaling pathways such as cell adhesion,migration,surface receptor activation,and immune regulation.Clinical results confirmed the correlation of neutrophil CD62L expression with the severity and prognosis of sepsis.The number of subpopulations expressing CD62L in peripheral blood neutrophils in the sepsis group was higher than that in the control group,but the expression level of CD62L in single cells in the sepsis group was significantly lower than that in the control group(P<0.01).Protein-protein interaction network analysis showed strong interaction between CD62L and multiple important proteins such as P-selectin,P-selectin ligand,E-selectin,and vascular cell adhesion molecule-1.ConclusionThere is heterogeneity in the surface markers o

关 键 词：脓毒症 中性粒细胞 异质性 质谱流式 CD62L 

分 类 号：R459.7[医药卫生—急诊医学]