高海拔地区H型高血压患者MTHFR C677T基因多态性和氧化应激水平的关系  

Relationship between MTHFR C677T gene polymorphism and oxidative stress levels in patients with H-type hypertension at high altitude

作　　者：张柠 樊世明[2] 任明[2] 普措 窦小红[2] 沈有录[2] 马艳梅[2] 薛睿 王福彦 黄强 鲁星琴 林莹 张吉辉 田辉珍 韩玉娟 韩淑萍 刘青灵 韩萍 卢天龙[2] 芦永良 鄂维建 ZHANG Ning;FAN Shiming;REN Ming;PU Cuo;DOU Xiaohong;SHEN Youlu;MA Yanmei;XUE Rui;WANG Fuyan;HUANG Qiang;LU Xingqin;LIN Ying;ZHANG Jihui;TIAN Huizhen;HAN Yujuan;HAN Shuping;LIU Qingling;HAN Ping;LU Tianlong;LU Yongliang;E Weijian(Graduate School of Qinghai University,Xining,810001,China;Department of Cardiology,Affiliated Hospital of Qinghai University)

机构地区：[1]青海大学研究生院,西宁810001 [2]青海大学附属医院心血管内科

出　　处：《临床心血管病杂志》2025年第3期196-201,共6页Journal of Clinical Cardiology

基　　金：青海省中央引导地方科技发展资金项目(No:2024ZY023);青海大学附属医院科学创新基金项目(No:FYXC-2017-12)。

摘　　要：目的:分析高海拔地区Hcy及其代谢酶MTHFR C677T与氧化应激水平的关系。方法:随机选取玉树地区(海拔3800~4200 m)H型高血压患者30例(H型高血压组)、非H型高血压患者30例(非H型高血压组)和健康受试者30例(健康对照组),以及西宁地区(海拔2260 m)H型高血压患者30例(H型高血压组)、非H型高血压患者30例(非H型高血压组)和健康受试者30例(健康对照组)。检测受试者血压、Hcy、氧化型谷胱甘肽(GSSG)、一氧化氮(NO)、超氧化物歧化酶(SOD)水平及亚甲基四氢叶酸还原酶(MTHFR C667T)基因型。结果:高Hcy水平患者具更高GSSG水平(P<0.05);MTHFR C677T突变基因型患者具有更高的SOD、NO水平(TT型>CT型>CC型)(P<0.05);Hcy与NO水平呈负相关性(r=-0.712,P<0.01),SOD与C677T基因型突变呈正相关(r=0.707,P<0.01),NO、Hcy与C677T基因型突变均呈正相关(r=0.642、0.452,P<0.05)。高海拔地区人群具更高的氧化应激和Hcy水平,更低的C677T突变基因型频率。结论:Hcy水平和MTHFR C677T突变基因型与抗氧化水平呈正相关性。Objective To analyse the relationship between Hcy and its metabolic enzyme MTHFR C677T and the level of oxidative stress at high altitude.Methods In this study,30 patients with H-type hypertension(H-type hypertension group),30 patients with non-H-type hypertension(non-H-type hypertension group),and 30 healthy subjects(healthy control group)were randomly selected from Yushu area(3800-4200 m above sea level),and 30 patients with H-type hypertension(H-type hypertension group),30 patients with non-H-type hypertension(non-H-type hypertension group),and 30 healthy subjects(healthy control group)were randomly selected from Xining area(2260 m above sea level).Blood pressure,Hcy,oxidised glutathione(GSSG),nitric oxide(NO),superoxide dismutase(SOD)levels,and methylenetetrahydrofolate reductase(MTHFR C667T)genotype were measured.Results Patients with high Hcy levels exhibited higher GSSG levels(P<0.05),patients with the MTHFR C677T mutant genotype had higher SOD and NO levels(TT type>CT type>CC type,P<0.05).Hcy levels were negatively correlated with NO levels(r=-0.712,P<0.01),SOD was positively correlated with the C677T genotype mutation(r=0.707,P<0.01),and both NO and Hcy levels were positively correlated with the C677T genotype mutation(r=0.642,0.452,P<0.05).Populations in high-altitude regions exhibited higher oxidative stress and Hcy levels,along with a lower frequency of the C677T mutant genotype.Conclusion Hcy levels and MTHFR C677T mutant genotypes are positively correlated with antioxidant levels.

关 键 词：H型高血压 MTHFR基因多态性 高海拔 氧化应激 

分 类 号：R544.1[医药卫生—心血管疾病]