芦丁促进人乳牙牙髓干细胞的增殖及成骨分化  

Rutin promoting the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth

作　　者：冯妮[1] 李月红 FENG Ni;LI Yuehong(Department of Stomatology,The Fourth Hospital of Changsha,Changsha 410006,China)

机构地区：[1]长沙市第四医院口腔科,长沙410006

出　　处：《中国医科大学学报》2025年第4期369-374,共6页Journal of China Medical University

基　　金：湖南创新型省份建设专项(2020ZK4073)。

摘　　要：目的探讨芦丁对人乳牙牙髓干细胞(SHEDs)增殖及成骨分化的影响及其与Janus蛋白酪氨酸激酶2(JAK2)/信号转导及转录激活蛋白3(STAT3)通路的调节关系。方法体外分离培养SHEDs,经鉴定后将SHEDs随机分为control组(正常培养),芦丁低、中、高剂量组(0.1、0.5、1μmol/L)和抑制剂组(1μmol/L芦丁+10μmol/L JAK2/STAT3通路抑制剂AG490),茜素红染色检测矿化结节数量,MTT法检测细胞增殖情况,试剂盒检测细胞碱性磷酸酶(ALP)活性,实时定量PCR检测Runt相关转录因子2(Runx2)、骨钙素(OCN)、碱性磷酸酶(ALP)mRNA表达,Western blotting检测Runx2、OCN、ALP及JAK2/STAT3信号通路蛋白的表达。结果与control组相比,芦丁低、中、高剂量组SHEDs光密度值、矿化结节数量、ALP活性、Runx2、OCN、ALP mRNA和蛋白水平、p-JAK2/JAK2、p-STAT3/STAT3显著升高(P<0.05);JAK2/STAT3通路抑制剂AG490减弱了芦丁对SHEDs增殖和成骨分化能力的增强作用(P<0.05)。结论芦丁可促进SHEDs增殖和成骨分化,其机制与激活JAK2/STAT3信号通路有关。Objective To investigate the effects of rutin on the proliferation and osteogenic differentiation of stem cells from human exfoliated deciduous teeth(SHEDs)and its regulatory relationship with the Janus protein tyrosine kinase 2(JAK2)/signal transducer and activator transcription 3(STAT3)pathway.Methods SHEDs were isolated and cultured in vitro.After identification,SHEDs were randomly grouped into the control group(normal culture),low,medium,and high dose rutin groups(0.1,0.5,1μmol/L),and the inhibitor group(1μmol/L of rutin+10μmol/L of the JAK2/STAT3 pathway inhibitor AG490).Alizarin red staining was applied to detect the number of mineralized nodules,and the MTT method was utilized to detect cell proliferation.A commercial kit was used to detect cell alkaline phosphatase(ALP)activity,and quantitative real-time PCR was applied to detect the mRNA expression of Runt-related transcription factor 2(Runx2),osteocalcin(OCN),and alkaline phosphatase(ALP).Western blotting was used to detect the expression of Runx2,OCN,ALP,and JAK2/STAT3 signaling pathway proteins.Results Compared to that of the control group,the optical density value of SHEDs cells,the number of mineralized nodules,ALP activity,Runx2,OCN,ALP mRNA and protein levels,and p-JAK2/JAK2 p-STAT3/STAT3 were all markedly increased in the low,medium,and high dose rutin groups(P<0.05).Treatment with the JAK2/STAT3 pathway inhibitor AG490 attenuated the enhancement of rutin on the proliferation and osteogenic differentiation of SHEDs(P<0.05).Conclusion Rutin promotes the proliferation and osteogenic differentiation of SHEDs,and its mechanism is related to the activation of the JAK2/STAT3 signaling pathway.

关 键 词：芦丁 Janus蛋白酪氨酸激酶2 信号转导及转录激活蛋白3 人乳牙牙髓干细胞 增殖 成骨分化 

分 类 号：R459.9[医药卫生—治疗学]