华北落叶松纯林和混交林土壤细菌群落β多样性及其环境解释  

Soil bacterial beta diversity and its environmental interpretation in pure and mixed stands of Larix principis-rupprechtii

作　　者：牛亚洁 李新 韩有志[4] 梁文俊[4] 王传旭[1,2] 王卓 NIU Yajie;LI Xin;HAN Youzhi;LIANG Wenjun;WANG Chuanxu;WANG Zhuo(Life Sciences Department,Yuncheng University,Yuncheng,Shanxi 044000,China;Shanxi Key Laboratory of Yuncheng Salt Lake Ecological Protection and Resource Utilization,Yuncheng,Shanxi 044000,China;Department of Biology,Xinzhou Teachers University,Xinzhou,Shanxi 034000,China;College of Forestry,Shanxi Agricultural University,Taigu,Shanxi 030801,China)

机构地区：[1]运城学院生命科学系,山西运城044000 [2]运城盐湖生态保护与资源利用厅市共建山西省重点实验室,山西运城044000 [3]忻州师范学院生物系,山西忻州034000 [4]山西农业大学林学院,山西太谷030801

出　　处：《森林与环境学报》2025年第3期235-245,共11页Journal of Forest and Environment

基　　金：国家自然科学基金项目“基于森林空间结构的华北落叶松人工林种子萌发及幼苗成活机制研究”(3150030508);山西省基础研究计划项目(自由探索类)“运城盐湖可培养嗜盐古菌的多样性及功能菌株筛选”(202203021211114);山西省科技创新人才团队“嗜盐微生物资源利用”(202204051001035);山西省高校科技创新项目“基于iCAMP零模型的运城盐湖湿地土壤微生物群落构建机制研究”(2024L337);运城市科技计划面上项目“运城盐湖湿地土壤环境-微生物-植被互馈作用机制研究”(YCKJ-2024030);运城市科技计划社会发展类项目“运城盐湖极端生境微生物‘暗物质’资源发掘与利用”(YCKJ-2024022)。

摘　　要：华北落叶松是黄土高原中东部典型生态脆弱区的造林先锋树种,深入理解其林下土壤细菌群落β多样性及其环境解释可为山地生态系统的生物多样性保护和生态管理提供理论支撑。运用Illumina高通量测序技术,以华北落叶松林区6个海拔梯度(1720、1850、1950、2050、2150、2250 m)不同林分类型表层(0~10 cm)和亚表层(10~20 cm)土壤为对象,研究细菌群落β多样性及其环境解释。结果表明:林分类型对土壤细菌群落β多样性的影响强于海拔和土层深度,且华北落叶松纯林中土壤细菌群落的β多样性高于华北落叶松-白桦混交林;土壤环境因子(主要是碱解氮和全氮)是影响纯林下土壤细菌群落β多样性的关键因素,总效应值和直接效应值均为0.378,为正影响,其次为土层深度;土壤环境因子(主要是容重和全磷)是影响混交林下土壤细菌群落β多样性的主要因素,其次是凋落物未分解层厚度,总效应值分别为-0.890和0.353。Larix principis-rupprechtii is the pioneer tree species for afforestation in the east-central Loess Plateau,China.Revealing the patterns of soil bacterial beta diversity in the stands of this species and its interpretation can provide theoretical support for biodiversity conservation and ecological management in mountain ecosystems.Illumina high-throughput sequencing was employed to investigate the bacterial beta diversity in surface(0-10 cm)and sub-surface(10-20 cm)soil layers of different stand types across six altitudinal gradients(1720,1850,1950,2050,2150,and 2250 m)in L.principis-rupprechtii forests in Lüliang Mountains.The results showed that stand types had stronger effect on soil bacterial beta diversity than altitude and soil depth.Soil bacterial beta diversity was higher in pure stands than in mixed stands(L.principis-rupprechtii-Betula platyphylla).Soil factors(alkaline-hydrolyzable nitrogen and total nitrogen)were the key factors influencing the soil bacterial beta diversity in pure stands,with the positive total and direct effect values both of 0.378,followed by soil layer depth.Soil factors(bulk density and total phosphorus)were the major factors influencing the soil bacterial beta diversity in mixed stands,followed by thickness of undecomposed litter layer,with total effect values of-0.890 and 0.353,respectively.

关 键 词：华北落叶松 土壤细菌群落 Β多样性 海拔 林分类型 

分 类 号：S718.5[农业科学—林学]