不同类型杉木代谢物差异及其机制分析  

Differences and mechanisms in metabolites among different types of Cunninghamia lanceolata

作　　者：昌婷 黄炳坤 王佩兰[1] 肖粲 吴炜 朱宁华[1] CHANG Ting;HUANG Bingkun;WANG Peilan;XIAO Can;WU Wei;ZHU Ninghua(College of Forestry,Central South University of Forestry and Technology,Changsha,Hunan 410004,China;Chenshan Forest Farm in Anfu County,Anfu,Jiangxi 343214,China;College of Furniture,Jiangxi Environmental Engineering Vocational College,Ganzhou,Jiangxi 341000,China;Jiangle State-Owned Forest Farm in Fujian Province,Jiangle,Fujian 353300,China)

机构地区：[1]中南林业科技大学林学院,湖南长沙410004 [2]安福县国营陈山林场,江西安福343214 [3]江西环境工程职业学院家具学院,江西赣州341000 [4]福建省将乐国有林场,福建将乐353300

出　　处：《森林与环境学报》2025年第3期307-317,共11页Journal of Forest and Environment

基　　金：2024年中央财政林草科技推广示范资金项目“新品种‘铁心杉’高效繁育及定向栽培技术推广与示范”(〔2024〕XT10号)。

摘　　要：为阐明不同类型杉木的材质差异形成机制,探讨了铁心杉(TX)、红心杉(HX)、速生杉(SS)3种不同类型杉木代谢物组成及含量差异,利用非靶向代谢组学技术测定铁心杉、红心杉、速生杉的代谢物组成和丰度,并结合差异代谢物富集分析及多元统计学方法,对代谢物进行功能注释、鉴定和差异性分析。主成分和相关性分析结果表明,铁心杉的代谢物与红心杉、速生杉存在显著差异,而红心杉与速生杉代谢物组成和丰度具有一定的相似性。铁心杉与红心杉、铁心杉与速生杉、红心杉与速生杉之间分别检测到1432、1121、1087个差异代谢物,其中168个为共有差异代谢物。KEGG富集分析显示,差异代谢物主要富集于类黄酮生物合成、花生四烯酸代谢、二萜生物合成等代谢途径,且类黄酮生物合成途径在铁心杉与红心杉、速生杉的差异代谢物中均显著富集。此外,视黄酰β-葡萄糖醛酸苷和2-(4-羟基苯基)-3,6-二甲氧基-8,8-二甲基-4H,8H-苯并[1,2-b:3,4-b′]二吡喃-4-酮在铁心杉与红心杉、速生杉比较组合中均显著上调。视黄酰β-葡萄糖醛酸苷和2-(4-羟基苯基)-3,6-二甲氧基-8,8-二甲基-4H,8H-苯并[1,2-b:3,4-b′]二吡喃-4-酮可能是区分铁心杉与其他类型杉木木材材质的重要标志代谢物。To explore the mechanisms underlying the characteristic differences in wood properties among different types of Cunninghamia lanceolata,this study compared the metabolites and their content among iron-heart C.lanceolata(TX),red-heart C.lanceolata(HX),and fast-growing C.lanceolata(SS).Non-targeted metabolomics was employed to determine the metabolite composition and abundance of TX,HX,and SS.Enrichment analysis and multivariate analysis were performed to annotate,identify,and compare the metabolites.The results of the principal component analysis and correlation analysis showed that the metabolites of TX had significant differences from those of HX and SS,while the metabolite composition and abundance had similarities between HX and SS.A total of 1432(TX vs HX),1121(TX vs SS),and 1087(HX vs SS)differential metabolites were identified,among which 168 differential metabolites were common.The KEGG enrichment analysis results showed that the differential metabolites were enriched in pathways such as flavonoid biosynthesis,arachidonic acid metabolism,and diterpenoid biosynthesis.The differential metabolites of TX vs HX and TX vs SS were significantly enriched in the flavonoid biosynthesis pathway.Retinylβ-glucuronide and 2-(4-hydroxyphenyl)-3,6-dimethoxy-8,8-dimethyl-4H,8H-benzo[1,2-b:3,4-b′]-dihydropyran-4-one were significantly upregulated in TX vs HX and TX vs SS.Retinylβ-glucuronide and 2-(4-hydroxyphenyl)-3,6-dimethoxy-8,8-dimethyl-4H,8H-benzo[1,2-b:3,4-b′]-dihydropyran-4-one may be important metabolites that distinguish the wood material of TX from other types of C.lanceolata.

关 键 词：差异代谢物 杉木 非靶向代谢组学 KEGG富集分析 

分 类 号：S718.5[农业科学—林学]