胶质母细胞瘤中LY86-AS1和KHDRBS2低表达与免疫细胞浸润及预后相关性分析  

Correlation analysis of low expression of LY86-AS1 and KHDRBS2 with immune cell invasion and prognosis in glioblastoma

作　　者：王莎莎 赵文浩 何锡宁 张杨杨[1] 常文丽 WANG Shasha;ZHAO Wenhao;HE Xining;ZHANG Yangyang;CHANG Wenli(Department of Pathology,Affiliated Hospital of Binzhou Medical University,Binzhou 256600;Department of Neurosurgery,Affiliated Binzhou People’s Hospital,Shandong First Medical University,Binzhou 256600,China)

机构地区：[1]山东省滨州医学院附属医院病理科,山东滨州256600 [2]山东省第一医科大学附属滨州市人民医院神经外科,山东滨州256600

出　　处：《细胞与分子免疫学杂志》2025年第3期245-253,共9页Chinese Journal of Cellular and Molecular Immunology

基　　金：滨州医学院科技计划项目(BY2020KJ04,BY2020KJ06)。

摘　　要：目的 探究淋巴细胞抗原86反义RNA 1(LY86-AS1)和含KH结构域的RNA信号转导相关分子2(KHDRBS2)在胶质母细胞瘤(GBM)中的表达、相关性及其对患者预后和免疫细胞浸润的影响。方法 基于基因表达综合(GEO)数据库中GSE50161数据集,采用加权基因共表达网络分析(WGCNA)和基因差异表达分析鉴定与GBM发生密切相关的LY86-AS1和KHDRBS2。采用癌症基因组图谱(TCGA)和中国胶质瘤基因组图谱(CGGA)数据库分析LY86-AS1和KHDRBS2表达与GBM患者预后的关系。采用多个数据集分析LY86-AS1和KHDRBS2表达的相关性及其与免疫细胞浸润的关系。采用实时定量PCR验证LY86-AS1和KHDRBS2在GBM和正常脑组织中的表达,人类蛋白质图谱(HPA)数据库获取KHDRBS2的蛋白表达及免疫组织化学染色验证KHDRBS2的蛋白表达。结果 LY86-AS1和KHDRBS2在GBM组织中低表达,且与GBM发生密切相关,两者呈显著正相关关系。LY86-AS1和KHDRBS2低表达组的患者总体生存率低于高表达组。LY86-AS1与初始B细胞、浆细胞、活化自然杀伤(NK)细胞、M1型巨噬细胞、活化肥大细胞和单核细胞呈显著正相关关系;KHDRBS2与初始B细胞、浆细胞、辅助T细胞、活化NK细胞和单核细胞呈显著正相关关系。结论 LY86-AS1和KHDRBS2在GBM中低表达,且与预后不良相关,影响肿瘤免疫微环境并可能作为GBM诊断和判断患者预后新的生物标志物。Objective To investigate the expression and correlation of LY86-AS1 and KHDRBS2 in glioblastoma(GBM),and their impacts on the prognosis of patients and immune cell infiltration.Methods Based on the GSE50161 dataset from the Gene Expression Omnibus(GEO)database,LY86-AS1 and KHDRBS2,which are closely related to the development of GBM,were identified by WGCNA and differential expression analysis.The Cancer Genome Atlas(TCGA)and the Chinese Glioma Genome Atlas(CGGA)databases were used to analyze the relationship between the expression of LY86-AS1 and KHDRBS2 and the prognosis of GBM patients.Multiple datasets were employed to analyze the correlation between the expression levels of LY86-AS1 and KHDRBS2 and its relationship with immune cell infiltration.Real-time quantitative PCR was used to verify the expression of LY86-AS1 and KHDRBS2 in GBM and normal brain tissues.The Human Protein Atlas(HPA)database was accessed to obtain the protein expression of KHDRBS2,and immunohistochemical staining was conducted to verify the protein expression of KHDRBS2.Results LY86-AS1 and KHDRBS2 were lowly expressed in GBM tissues and were closely related to the development of GBM,showing a significant positive correlation.Patients with low expression levels of LY86-AS1 and KHDRBS2 had a lower overall survival rate than those with high expression levels. LY86-AS1 was positively correlated with naive B cells, plasma cells, activated NK cells, M1 macrophages, activated mast cells and monocytes. KHDRBS2 was positively correlated with naive B cells, plasma cells, helper T cells, activated NK cells and monocytes. Conclusion The low expression levels of LY86-AS1 and KHDRBS2 in GBM, which is associated with poor prognosis, affect the tumor immune microenvironment and may serve as potential new biomarkers for the diagnosis of GBM and the prognosis assessment of patients.

关 键 词：胶质母细胞瘤(GBM) 长链非编码RNA LY86-AS1 KHDRBS2 免疫细胞浸润 预后分析 

分 类 号：R392[医药卫生—免疫学] R739.41[医药卫生—基础医学]