暗紫贝母茎化学成分研究  

Chemical constituents from the stems of Fritillaria unibracteata

作　　者：李敏 米雅慧 蒯海敏 胡晓龙 汪豪[1] LI Min;MI Yahui;KUAI Haimin;HU Xiaolong;WANG Hao(School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 211198,China)

机构地区：[1]中国药科大学中药学院,南京211198

出　　处：《中国药科大学学报》2025年第2期160-165,共6页Journal of China Pharmaceutical University

基　　金：云南省重大科技专项(202402AA310001);云南省专家工作站项目(202305AF150096);迪庆州-中国药科大学州校合作项目(2021ZXYD01)。

摘　　要：采用硅胶及制备HPLC等柱色谱方法对暗紫贝母(Fritillaria unibracteata P.K.Hsiao&K.C.Hsia)茎乙醇提取物的化学成分进行分离纯化,通过NMR、ESI-MS等波谱技术鉴定化合物结构,分离鉴定了9个化合物,分别为:27-hydroxychlorogenone(1),sieboldogenin(2),(3β,25S)-spirost-5-ene-3,17,27-triol(3),拉肖皂苷元(4),替告皂苷元酮(5),啤酒甾醇(6),过氧化麦角甾醇(7),豆甾醇(8),β-谷甾醇(9)。其中化合物1为新化合物,化合物2~9均为首次从暗紫贝母茎中分离得到。体外活性初步评价了化合物1~9对A549细胞的细胞毒活性,结果显示,化合物6具有中等强度的细胞毒活性,IC50为(14.16±1.11)μmol/L。Chemical investigation of the stems of Fritillaria unibracteata P.K.Hsiao&K.C.Hsia resulted in the isolation of nine compounds,by means of silica gel column chromatography,and preparative HPLC.Based on spectroscopic and chemical evidence,these compounds were identified as:27-hydroxychlorogenone(1),sieboldogenin(2),(3β,25S)-spirost-5-ene-3,17,27-triol(3),laxogenin(4),tigogenone(5),cerevisterol(6),ergosterol peroxide(7),stigmaterol(8),and β-sitosterol(9).Compound 1 was a new compound,and compounds 2-9 were isolated from the stems of Fritillaria unibracteata for the first time.The inhibitory effects of compounds 1−9 on A549 cells were determined using the MTT method.The results show that compound 6 exhibits moderate inhibitory activity with an IC50 value of(14.16±1.11)μmol/L.

关 键 词：暗紫贝母 茎 甾体 化学成分 细胞毒 

分 类 号：R284.1[医药卫生—中药学]