汉黄芩素对Aβ_(1-42)和D-半乳糖诱导的小鼠学习记忆损害的改善作用  

Wogonin ameliorates Aβ_(1-42) and D-galactose-induced learning and memory impairment in mice

作　　者：章琪露 聂睿哲 魏立彬 郭青龙 唐苏苏 ZHANG Qilu;NIE Ruizhe;WEI Libin;GUO Qinglong;TANG Susu(Department of Pharmacology,School of Pharmacy,China Pharmaceutical University,Nanjing 211198;Department of Physiology,School of Basic Medicine and Clinical Pharmacy,China Pharmaceutical University,Nanjing 211198;Shandong Provincial Key Laboratory of Polymorphic Drugs,Shandong Yikang Pharmaceutical Co.,Ltd.,Tengzhou 277500,China)

机构地区：[1]中国药科大学药学院药理系,南京211198 [2]中国药科大学基础医学与临床药学学院生理学系,南京211198 [3]山东益康药业股份有限公司,山东省晶型药物重点实验室,滕州277500

出　　处：《中国药科大学学报》2025年第2期207-215,共9页Journal of China Pharmaceutical University

基　　金：山东省中央引导地方科技发展资金项目(YDZX2023082);泰山产业领军人才工程;枣庄英才集聚工程基金项目:山东省枣庄英才计划(2021年,产业创新类);泰山产业创新领军人才;山东省技术创新引导计划(中央引导地方科技发展资金)项目(YDZX2023082)。

摘　　要：研究汉黄芩素对小鼠学习记忆的改善作用。分别采用侧脑室注射Aβ_(1-42)建立小鼠学习记忆损害模型与腹腔注射D-半乳糖建立小鼠急性衰老模型,连续28 d灌胃给予75、150和300 mg/kg汉黄芩素,采用Morris水迷宫、新物体识别、旷场实验检测汉黄芩素对小鼠学习记忆的影响。结果显示,在Aβ_(1-42)诱导的学习记忆损害模型中,与模型组相比,150 mg/kg和300 mg/kg汉黄芩素给药组小鼠在新物体识别实验中对新物体的识别指数显著增加;150 mg/kg汉黄芩素给药组小鼠在Morris水迷宫实验测试期目标象限停留时间百分比显著增加,而在旷场实验中各组小鼠的运动总距离无显著变化。在D-半乳糖诱导的急性衰老模型中,与模型组相比,150 mg/kg汉黄芩素组小鼠在Morris水迷宫实验测试期穿越平台次数显著增加,75、150和300mg/kg汉黄芩素组小鼠在Morris水迷宫实验测试期目标象限停留时间百分比显著增加;而在旷场实验中各组小鼠的运动总距离无显著变化。研究提示汉黄芩素对Aβ_(1-42)和D-半乳糖诱导的小鼠学习记忆损害均具有改善作用,并对小鼠自发活动无显著影响。此外,采用Western blot实验检测发现,汉黄芩素可显著减少Aβ_(1-42)和D-半乳糖诱导的小鼠海马区神经细胞凋亡。这些结果表明,汉黄芩素可能对阿尔茨海默病和衰老相关的学习记忆损害具有改善作用。To investigate the effects of Wogonin(WO)on learning and memory impairment,Aβ_(1-42) was injected intracerebroventricularly to induced a mouse learning and memory impairment model,and D-galactose was injected intraperitoneally to induced a mouse acute aging model.Mice were administered WO(75,150,or 300 mg/kg)by oral gavage for 28 consecutive days.Cognitive function was assessed using the Morris water maze(MWM),novel object recognition(NOR),and open field tests(OFT).In the Aβ_(1-42) model,WO treatment(150 and 300 mg/kg)significantly improved the recognition index in the NOR test,while the 150 mg/kg group showed increased target quadrant preference in the MWM test.No changes in the total distance traveled in OFT.In the D-galactose aging model,the 150 mg/kg WO group exhibited increased platform crossings in the MWM test,and all WO doses(75,150,and 300 mg/kg)enhanced target quadrant preference,with no alterations in spontaneous movement.Western blot analysis revealed that WO significantly attenuated hippocampal apoptosis in both models.These findings suggest that WO ameliorates learning and memory impairment associated with Alzheimer’s disease and aging.

关 键 词：汉黄芩素 Aβ_(1-42) D-半乳糖 记忆损害 细胞凋亡 

分 类 号：R965[医药卫生—药理学]