柴胡皂苷D改善抗癌药物环磷酰胺诱导的免疫功能低下  

Saikosaponin D Ameliorating Immunosuppression Induced by

作　　者：陈云曦 刘夫锋 CHEN Yunxi;LIU Fufeng(College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《天津科技大学学报》2025年第2期22-28,36,共8页Journal of Tianjin University of Science & Technology

基　　金：天津市自然科学基金项目(18JCZDJC33000)。

摘　　要：为了探究柴胡皂苷D(SSD)对免疫低下小鼠的影响,以环磷酰胺(CTX)诱导的免疫低下小鼠为研究对象,观察不同剂量SSD对小鼠胸腺和脾脏指数、血细胞数量、淋巴细胞和巨噬细胞功能、血清IgG和IgM浓度、脾细胞CD4^(+)和CD8^(+)T细胞比例和细胞凋亡率、脾脏结构以及JAK2/STAT3信号通路的影响。结果表明:除了红细胞和血小板数量外,CTX组各项指标均显著降低(P<0.05),脾脏结构被破坏且细胞凋亡增多;各组SSD均可明显改善脾脏结构,提高CD4^(+)T细胞比例并降低细胞凋亡率。低剂量组胸腺指数、白细胞数和p-STAT3表达显著增加(P<0.05)。高剂量组体重、白细胞数、IgG水平、T细胞和B细胞增殖指数、CD8^(+)T细胞比例以及JAK2/STAT3通路相关蛋白表达均明显上升(P<0.05)。因此,SSD可以显著增强CTX诱导的免疫低下小鼠的免疫功能,机制可能与JAK2/STAT3通路的激活有关。In order to explore the effect of saikosaponin D(SSD)on immunocompromised mice,the immunosuppressed mice induced by cyclophosphamide(CTX)were used to observe the effects of different doses of SSD on thymus and spleen indexes,blood cell counts,lymphocyte proliferation and macrophage function,serum IgG and IgM concentration,the ratio of CD4^(+)and CD8^(+)T cells and spleen cell apoptosis rate,structure of spleen and expression of JAK2/STAT3 signaling path-way.The results showed that except for the number of erythrocyte and thrombocyte,all indexes in CTX group were decreased significantly(P<0.05),spleen structure was destroyed and apoptosis was promoted.SSD could significantly improve the spleen structure,increase the proportion of CD4^(+)T cells and reduce the apoptosis.The thymus index,leukocyte count and p-STAT3 expression were increased significantly in the low-dose SSD group(P<0.05),while the body weight,leukocyte count,IgG level,T cell and B cell proliferation indexes,the ratio of CD8^(+)T cells and JAK2/STAT3 signaling ex-pression were increased significantly in the high-dose SSD group(P<0.05).Therefore,SSD could effectively enhance the immune function of CTX-induced immunocompromised mice,and the mechanism might be associated with the activation of JAK2/STAT3 pathway.

关 键 词：柴胡皂苷D 环磷酰胺 免疫低下 JAK2/STAT3 

分 类 号：R392[医药卫生—免疫学]