一种超氧阴离子荧光探针的合成与应用  

Synthesis and application of a superoxide anion fluorescent probe

作　　者：王露露 时杰 王兰 后华清 WANG Lulu;SHI Jie;WANG Lan;HOU Huaqing(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230601,China)

机构地区：[1]合肥工业大学食品与生物工程学院,安徽合肥230601

出　　处：《合肥工业大学学报(自然科学版)》2025年第4期512-517,523,共7页Journal of Hefei University of Technology:Natural Science

基　　金：安徽省自然科学基金资助项目(2202085J04)。

摘　　要：文章以咖啡酸基团作为超氧阴离子(O_(2)^(-)·)识别位点合成了一种吩噻嗪类荧光探针PTZ-C,通过荧光开启的方式来检测O_(2)^(-)·。在O_(2)^(-)·存在的情况下,PTZ-C中咖啡酸残基的给电子邻苯二酚可以被氧化形成吸电子的邻苯二醌,发生荧光共振能量转移,在470 nm处出现新峰,并且在紫外灯下观察到溶液由无色荧光变为蓝色荧光,响应时间在5 s内。PTZ-C探针能够特异性识别O_(2)^(-)·,抗干扰能力较强,pH值适用范围较广,检测限为12.8 nmol/L,且对交替加入O_(2)^(-)·和抗坏血酸的氧化还原过程表现出良好的可逆性。此外,该探针的细胞毒性小,可应用于巨噬细胞RAW264.7的内源性O_(2)^(-)·荧光成像。In this paper,a phenothiazine fluorescent probe PTZ-C was synthesized using caffeic acid group as the recognition site of superoxide anion(O_(2)^(-)·),and O_(2)^(-)·was detected by fluorescence activation.In the presence of O_(2)^(-)·,the electron-donating pyrocatechol of caffeic acid residues in PTZ-C can be oxidized to form electron-withdrawing o-benzoquinone,and the fluorescence resonance energy transfer occurs.A new peak appears at 470 nm,and the solution is observed to change from colorless fluorescence to blue fluorescence under ultraviolet lamp,and the response speed is within 5 s.Probe PTZ-C can specifically recognize O_(2)^(-)·,has strong anti-interference ability and a wide range of pH value,and the detection limit is 12.8 nmol/L.It shows good reversibility to the redox process of alternately adding O_(2)^(-)·and ascorbic acid.In addition,it shows little cytotoxicity,and can be applied in endogenic O_(2)^(-)·fluorescence imaging of macrophage RAW264.7.

关 键 词：超氧阴离子 吩噻嗪 咖啡酸 荧光探针 

分 类 号：Q811.5[生物学—生物工程]