HBeAg阴性慢性乙型肝炎患者血清HBV  

Analysis of the correlation between serum HBV DNA test results and liver fibrosis indicators in HBeAg-negative chronic hepatitis B patients

作　　者：杨清梅 YANG qing-mei(Laboratory Department of Xiamen Third Hospital,Fujian Xiamen 361100)

机构地区：[1]厦门市第三医院检验科,福建厦门361100

出　　处：《医学检验与临床》2025年第3期29-33,共5页Medical Laboratory Science and Clinics

摘　　要：目的:分析HBeAg阴性慢性乙型肝炎患者血清HBVDNA检验结果及其与肝纤维化指标的相关性。方法:选择厦门市第三医院2021年1月-2022年12月100例HBeAg阴性慢性乙型肝炎患者,依据患者是否合并肝纤维化分组,合并肝纤维化者54例为肝纤维化组,无肝纤维化者46例为无肝纤维化组,实时免疫荧光PCR检测血清HBV-DNA含量,采用化学发光法检测四项肝纤维化指标[层黏蛋白(LN)、透明质酸(HA)、Ⅳ型胶原(Ⅳ-C)、三型前胶原(PCⅢNP)]。比较两组血清HBV-DNA含量和肝纤维化指标,采用Pearson相关分析HBeAg阴性慢性乙型肝炎患者血清HBVDNA检验结果及其与肝纤维化指标的相关性。再将54例肝纤维化患者根据肝纤维化程度分为31例重度组和23例轻中度组,比较两组血清HBV-DNA含量,用Logistic回归分析血清HBV-DNA含量对肝纤维化及肝纤维化程度的影响。结果:肝纤维化组患者血清HBV-DNA含量、LN、HA、IV-C、PCINP水平高于无肝纤维化组,差异有统计学意义(P<0.05);HBeAg阴性慢性乙型肝炎患者血清HBV-DNA含量的和肝纤维化指标LN、HA、IV-C、PCIINP之间呈正相关(r>0,P<0.05);肝纤维化重度组患者血清HBV-DNA含量高于轻中度组,差异有统计学意义(P<0.05);Logistic回归分析检验结果显示,血清HBV-DNA含量高是肝纤维化及肝纤维化程度进展的影响因素(OR>1,P<0.05)。结论:HBeAg阴性慢性乙型肝炎患者血清HBVDNA检验结果及其与肝纤维化指标呈正相关,且影响肝纤维化的发生及肝纤维化程度进展。Objective:To analyze the serum HBV DNA test results of HBeAg-negative chronic hepatitis B patients and their correlation with liver fibrosis indicators.Methods:A total of 100 HBeAgnegative chronic hepatitis B patients from Xiamen Third Hospital from January 2021 to December 2022 were selected.Patients were divided into two groups based on whether they had liver fibrosis.54 patients with liver fibrosis were in the liver fibrosis group,and 46 patients without liver fbrosis were in the non-liver fibrosis group.The serum HBV-DNA content was detected by real-time immunofluorescence PCR,and four liver fibrosis indicators(lamin(LN)hyaluronic acid(HA),type IV collagen(IV-C),and type II procollagen(PC II NP)were detected by the chemiluminescence method.We compared the serum HBV DNA content and liver fibrosis indicators between two groups,and used Pearson correlation analysis to examine the serum HBV DNA test results of HBeAg negative chronic hepatitis B patients and their correlation with liver fibrosis indicators.54 patients with liver fibrosis were further divided into 31 severe groups and 23 mild to moderate groups based on the degree ofliver fibrosis.The serum HBV-DNA content of the two groups was compared,and the impact of serum HBV-DNA content on liver fibrosis and its degree was analyzed using logistic regression.Results:The serum HBV-DNA content,LN,HA,IV-C,and PC II NP levels in the liver fibrosis group were higher than those in the non-liver fibrosis group,and the differences were statistically significant(P<0.05).There is a positive correlation(r>0,P<0.05)between the serum HBV-DNA content and liver fibrosis markers LN,HA,IV-C,and PC II NP in HBeAg negative chronic hepatitis B patients;The serum HBV-DNA content in patients with severe liver fibrosis was higher than that in the mild to moderate group,and the difference was statistically significant(P<0.05);The results oflogistic regression analysis showed that high serum HBV-DNA content was a factor influencing liver fibrosis and its progression(OR≥1,P<0.05).Con

关 键 词：慢性乙型肝炎 肝纤维化 乙型肝炎病毒 

分 类 号：R512.6[医药卫生—内科学]