干旱胁迫对冷蒿生长、生理指标和药效物质含量的影响  

Impact of drought stress on the growth,physiological indices,and pharmacodynamic substance content of Artemisia frigida Willd.

作　　者：金昆 丛龙琦 穆东祺 曹阳 张奥 薛焱 JIN Kun;CONG Longqi;MU Dongqi;CAO Yang;ZHANG Ao;XUE Yan(School of Pharmacy,Inner Mongolia Medical University,Hohhot 010110,China)

机构地区：[1]内蒙古医科大学药学院,内蒙古呼和浩特010110

出　　处：《江苏农业学报》2025年第3期493-500,共8页Jiangsu Journal of Agricultural Sciences

基　　金：内蒙古自然科学基金面上项目(2022MS08014);内蒙古高等学校科学研究重点项目(NJZZ22647);内蒙古医科大学面上项目(YKD2023MS083)。

摘　　要：本研究以冷蒿(Artemisia frigida Willd.)为试验材料,分别探究轻度干旱胁迫处理(土壤相对含水率保持在75%±5%)、中度干旱胁迫处理(土壤相对含水率保持在50%±5%)、重度干旱胁迫处理(土壤相对含水率保持在25%±5%)对冷蒿生长发育、抗氧化酶活性、活性氧含量、抗氧化物含量和药用物质含量的影响。结果表明,随干旱胁迫程度的提高,冷蒿叶片游离脯氨酸(Pro)含量、还原型抗坏血酸(AsA)含量、还原型谷胱甘肽(GSH)含量整体呈上升趋势,谷胱甘肽还原酶(GR)、过氧化氢酶(CAT)活性呈上升趋势。表明在受到干旱胁迫后,冷蒿能够维持动态平衡和相对稳定的内环境。随干旱胁迫程度的提高,冷蒿叶片叶周长、叶面积、叶长、叶宽、株高、茎粗等生长指标整体呈下降趋势,总黄酮含量呈上升趋势。相关性分析结果表明,冷蒿株高、茎粗等生长指标与干旱胁迫程度呈显著负相关(P<0.05),总黄酮含量与干旱胁迫程度呈显著正相关(P<0.05)。对冷蒿产量-品质线性回归模型进行分析,结果表明在含水率为57%~59%的土壤中栽培冷嵩可以较好地平衡其产量与质量。本研究结果可为蒙药药材冷蒿的优质高效栽培提供理论依据。This study used Artemisia frigida Willd.as the experimental material to investigate the effects of mild drought stress(soil relative water content maintained at 75%±5%),moderate drought stress(soil relative water content maintained at 50%±5%),and severe drought stress(soil relative water content maintained at 25%±5%)on the growth and development of Artemisia frigida Willd.,as well as its antioxidant enzyme activities,reactive oxygen species content,antioxidant substance content and pharmacodynamic substance content.The results showed that with the increase in drought stress intensity,the overall contents of free proline(Pro),reduced ascorbic acid(AsA),and reduced glutathione(GSH)in the leaves of Artemisia frigida Willd.increased,and the activities of glutathione reductase(GR)and catalase(CAT)also increased.The above results indicated that after being subjected to drought stress,Artemisia frigida Willd.could maintain dynamic equilibrium and a relatively stable internal environment.As the drought stress intensity increased,the growth indicators of Artemisia frigida Willd.,such as leaf perimeter,leaf area,leaf length,leaf width,plant height,and stem diameter,showed an overall downward trend,while the total flavonoids content increased.The correlation analysis results showed that the growth indicators of Artemisia frigida Willd.,such as plant height and stem diameter,were significantly negatively correlated with drought stress intensity(P<0.05),while the total flavonoids content was significantly positively correlated with drought stress intensity(P<0.05).The analysis results of the yield-quality linear regression model of Artemisia frigida Willd.indicated that cultivating it in soil with a water content of 57%-59%could achieve a better balance between yield and quality.This study provides a theoretical basis for the high-quality and efficient cultivation of the Mongolian medicinal herb Artemisia frigida Willd..

关 键 词：干旱胁迫 冷蒿 阿给 生理指标 药效物质 

分 类 号：R931.2[医药卫生—生药学]