绿豆腐霉根腐病抗性鉴定技术体系构建及抗病资源筛选  

Establishment of resistance identification technology system for mung bean Pythium myriotylum root rot and screening of resistant resources

作　　者：闫强 刘欢 王瑞敏 薛冬 周琰琰 郑豫 武婷 任亚举 陈雨婷 刘欣悦 袁星星[1] 陈新[1] YAN Qiang;LIU Huan;WANG Ruimin;XUE Dong;ZHOU Yanyan;ZHENG Yu;WU Ting;REN Yaju;CHEN Yuting;LIU Xinyue;YUAN Xingxing;CHEN Xin(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China)

机构地区：[1]江苏省农业科学院经济作物研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京210014

出　　处：《江苏农业学报》2025年第3期510-516,共7页Jiangsu Journal of Agricultural Sciences

基　　金：国家食用豆产业技术体系生物防治与综合防控岗位科学家项目(CARS-08-G15);江苏省种业揭榜挂帅项目[JBGS(2021)004];种质资源精准鉴定评价项目(005012691230229)。

摘　　要：由群结腐霉(Pythium myriotylum)侵染引起的绿豆腐霉根腐病是近2年新报道的一种土传病害,该病原菌对绿豆具有强致病性。为了构建绿豆对群结腐霉的室内抗性评价技术,实现抗病资源的快速有效筛选,本研究使用灌根接种法、菌层接种法和下胚轴创伤接种法3种接种方法评价绿豆对群结腐霉的抗性并比较3种接种方法。结果表明,菌层接种法具有操作简便、试验周期短、结果稳定的优点。为筛选抗腐霉根腐病的绿豆种质资源,采用菌层接种法对收集到的394份绿豆种质资源进行了抗性鉴定。结果表明,98.48%的种质(388份)表现为中感及以上感病等级[病情指数(DI)>40.0],表现中抗的种质资源(20.0<DI≤40.0)有6份(占1.52%),没有发现抗病或免疫的种质资源(DI≤20.0),表明所收集的绿豆种质资源中具有群结腐霉抗性的种质资源较为缺乏。本研究结果为挖掘绿豆对腐霉根腐病的抗性资源提供了候选方法。The mung bean root rot caused by Pythium myriotylum is a recently reported soil-borne disease that presents a considerable pathogenic hazard to mung bean.To establish a laboratory technique for assessing resistance of mung bean against P.myriotylum and achieve rapid and effective screening of disease-resistant resources,three inoculation methods including root irrigation inoculation method,mycelial layer inoculation method and hypocotyl wound inoculation method were used to evaluate and compare the resistance of mung bean to P.myriotylum.The results showed that the mycelial layer inoculation method had the advantages of simple operation,short test period,and stable results.To screen mung bean germplasm resources with resistance to root rot disease caused by P.myriotylum,394 collected mung bean germplasm resources were assessed using the mycelial layer inoculation method.The results showed that 98.48%(388 samples)of germplasms exhibited medium to high susceptibility,with disease index(DI)>40.0,only six germplasms(1.52%)exhibited intermediate resistance(20.0<DI≤40.0),and none showed resistance or immunity(DI≤20.0).The results indicated that the collected mung bean germplasms with resistance to P.myriotylum were scarce.In this study,we established a potential method for identifying resistance resources of mung bean against root rot disease caused by Pythium species.

关 键 词：绿豆 群结腐霉 根腐病 抗性评价 抗病资源 

分 类 号：S522[农业科学—作物学]