筛选白花油茶胚性愈伤组织诱导成苗的关键激素通路及相关基因  

Screening of key hormone pathways and related genes for the induction of plantlets from embryogenic callus of Camellia oleifera

作　　者：王江英[1] 葛金涛[1] 邵小斌[1] 朱朋波[1] 孙明伟[1] 赵统利[1] 汤雪燕[1] WANG Jiangying;GE Jintao;SHAO Xiaobin;ZHU Pengbo;SUN Mingwei;ZHAO Tongli;TANG Xueyan(Leisure Agriculture Laboratory of Lianyungang Academy of Agricultural Sciences,Lianyungang 222000,China)

机构地区：[1]连云港市农业科学院休闲农业研究室,江苏连云港222000

出　　处：《江苏农业学报》2025年第3期599-614,共16页Jiangsu Journal of Agricultural Sciences

基　　金：连云港市财政专项(QNJJ2104)。

摘　　要：植物激素在植物无性繁殖器官再生中发挥着关键作用,本研究探究白花油茶再生过程中激素调控机理,为加速山茶属植物再生及遗传转化提供理论基础。本研究采用综合代谢组学-转录组学分析方法,筛选出白花油茶胚性愈伤组织诱导及分化成苗5个阶段(S1~S5)中植物激素相关的差异代谢物(DAMs)和编码关键代谢酶的差异表达基因(DEGs)。液相色谱-质谱(LC-MS)分析结果显示,在S1~S5共获得51种激素相关差异代谢物;通过Illumina HiSeq测序,共发现与植物激素相关的54个差异表达基因,其中21个差异表达基因作用于植物激素信号转导通路中,33个差异表达基因参与激素合成代谢。此外,KEGG富集分析结果表明,在白花油茶植物再生过程中,相比于其他激素,生长素、细胞分裂素和水杨酸的调控作用较为明显,激素含量分析结果表明,生长素和水杨酸的平均含量分别高达细胞分裂素的18.81倍和201.94倍,而赤霉素等其他激素仅为细胞分裂素的2%~27%;同时差异表达基因也显著表达在生长素、细胞分裂素和水杨酸的合成途径中。研究以上3种激素合成途径中的DAMs和DEGs之间的通路关系发现,在KEGG通路中共富集到8种差异代谢物,包括吲哚、L-色氨酸、色胺、吲哚-3-乙酸、玉米素核苷、反式-玉米素-9-β-葡萄糖苷、异戊烯腺嘌呤核苷、L-苯丙氨酸;18个差异表达基因,包括ALDH、TAA1、COMT、IPT、TRIT1、CKX、PAT和PAL等。对其他内源激素的进一步分析结果表明,赤霉素、脱落酸和茉莉酸在油茶再生过程中含量较低,生长素和水杨酸平均含量分别高达赤霉素的757倍和8130倍;同时KEGG富集了15个差异表达基因,包括KAO、GA2ox、ZEP及OPR等,这些基因在再生过程中与ALDH等18个基因发挥着相互作用。Plant hormones are crucial for the regeneration of plant’s asexual reproductive organs.The purpose of this study is to explore the mechanism of hormone regulation in the regeneration process of Camellia oleifera,and to provide a theoretical basis for accelerating the regeneration and genetic transformation of Camellia plants.In this research,an integrated metabolomic-transcriptomic analysis was employed to screen out the plant hormone-related differential accumulated metabolites(DAMs)and differentially expressed genes(DEGs)encoding key metabolic enzymes in five stages(S1,S2,S3,S4,S5)of embryogenic callus induction and differentiation into seedlings of Camellia oleifera.The results of liquid chromatography-mass spectrometry(LC-MS)analysis showed that a total of 51 hormone-related differential metabolites were obtained from S1 to S5.Through Illumina HiSeq sequencing,a total of 54 differentially expressed genes related to plant hormones were found,of which 21 differentially expressed genes acted on plant hormone signal transduction pathways,and 33 differentially expressed genes were involved in hormone synthesis and metabolism.In addition,the results of KEGG enrichment analysis indicated that the regulation of auxin,cytokinin and salicylic acid was more obvious than that of other hormones in the regeneration process of Camellia oleifera.The results of hormone content analysis revealed that the average contents of auxin and salicylic acid were as high as 18.81 times and 201.94 times of cytokinin,respectively,while the average contents of other hormones such as gibberellin were only 2%-27%of the average content of cytokinin.At the same time,the differentially expressed genes were also significantly expressed in the synthesis pathways of auxin,cytokinin and salicylic acid.By studying the pathway relationships between DAMs and DEGs in the above three hormone synthesis pathways,it was found that eight differential metabolites(such as indole,L-tryptophan,tryptamine,indole-3-acetic acid,zeatin nucleoside,trans-zeatin-9-�

关 键 词：油茶 植物激素 植株再生 代谢组 转录组 差异代谢物 差异表达基因 

分 类 号：S794.4[农业科学—林木遗传育种]