静水压诱导三倍体勃氏雅罗鱼的研究  

HYDROSTATIC INDUCTION OF TRIPLOID LEUCISCUS BRANDTI(DYBOWSKI)

作　　者：于涵 郭贵良 牛小天[1] 王桂芹[1] 陈秀梅[1] 邵云龙 宋健 孔祎頔 李民 YU Han;GUO Gui-Liang;NIU Xiao-Tian;WANG Gui-Qin;CHEN Xiu-Mei;SHAO Yun-Long;SONG Jian;KONG Yi-Di;LI Min(Jilin Agricultural University,Changchun 130000,China;Changchun Aquatic Product Quality and Safety Testing Center,Changchun 130000,China)

机构地区：[1]吉林农业大学,长春130118 [2]长春市水产品质量安全检测中心,长春130000

出　　处：《水生生物学报》2025年第5期95-101,共7页Acta Hydrobiologica Sinica

基　　金：吉林省科技发展计划(20240303067NC);国家特色淡水鱼产业技术体系(CARS-46)资助。

摘　　要：研究运用组织学和对比试验等方法筛选静水压诱导三倍体勃氏雅罗鱼的最适条件。研究表明:(1)受精后5min的组织切片,可见第二极体明显突出于受精卵卵膜,准备排出第二极体。(2)诱导起始时间5min的受精率与对照组无显著性差异(P>0.05),但显著高于其他试验组(P<0.05);起始时间4min和5min的孵化率无显著差异(P>0.05),但均显著低于对照组(P<0.05),显著高于其他试验组(P<0.05);起始时间5min的三倍体率显著高于其他试验组(P<0.05)。(3)静水压压强60 MPa的受精率与对照组之间无显著性差异(P>0.05),但显著高于其他试验组(P<0.05);65 MPa的孵化率显著低于其他各组(P<0.05),50、55和60 MPa之间的孵化率无显著性差异(P>0.05),但均显著低于对照组(P<0.05);60 MPa的三倍体率显著高于其他试验组(P<0.05)。(4)诱导持续时间4min与5min之间的受精率无显著性差异(P>0.05),但显著高于其他各试验组(P<0.05),显著低于对照组(P<0.05);各试验组的孵化率均显著低于对照组(P<0.05),持续时间4min的孵化率和三倍体率均显著高于其他试验组(P<0.05)。在实验条件下,当水温(17±1)℃时,三倍体勃氏雅罗鱼的最佳诱导条件为受精后5min,静水压强60 MPa,持续处理时间4min。In this study,the optimum conditions for hydrostatic induction of triploid Leuciscus brandti were selected by means of histological and comparative tests.The results showed that:(1)The tissue section examined 5min after fertilization showed that the second polar body was obviously protruding from the egg membrane of the fertilized egg,indicating its readiness for discharge.(2)The fertilization rate of 5min initiation time was not significantly different from the control group(P>0.05),but was significantly higher than that of other experimental groups(P<0.05).There was no significant difference in incubation rate between 4min and 5min(P>0.05),but it was significantly lower than that of the control group(P<0.05),and significantly higher than that of other experimental groups(P<0.05).The triploid rate of the initial time of 5min was significantly higher than that of other experimental groups(P<0.05).(3)There was no significant difference between the fertilization rate of hydrostatic pressure 60 MPa and control group(P>0.05),but it was significantly higher than that of other experimental groups(P<0.05).The hatching rate of 65 MPa was significantly lower than that of other groups(P<0.05),and the hatching rate of 50,55 and 60 MPa had no significant difference(P>0.05),but was significantly lower than that of the control group(P<0.05).The triploid rate of 60 MPa was significantly higher than that of other experimental groups(P<0.05).(4)There was no significant difference in fertilization rate between 4 and 5min induction duration(P>0.05),but it was significantly higher than that of other experimental groups(P<0.05),and significantly lower than that of the control group(P<0.05).The hatching rate of experimental groups was significantly lower than that of the control group(P<0.05),and the hatching rate and triploid rate lasting 4min were significantly higher than those of other experimental groups(P<0.05).Under the conditions of this experiment,with a water temperature of(17±1)℃,the best induction conditions for triploi

关 键 词：静水压 三倍体 倍性鉴定 诱导条件 勃氏雅罗鱼 

分 类 号：S965.1[农业科学—水产养殖]