饲料桑对盐碱胁迫的生理生化响应  

Physiological and biochemical responses of forage mulberry to saline-alkali stress

作　　者：贾晨阳 田婕 张通 李红姣[1,2] JIA Chenyang;TIAN Jie;ZHANG Tong;LI Hongjiao(College of Forestry,Hebei Agricultural University,Baoding 071000,China;Hebei Provincial Key Laboratory of Forest Germplasm Resources and Forest Protection,Baoding 071000,China)

机构地区：[1]河北农业大学林学院,河北保定071000 [2]河北省林木种质资源与森林保护重点实验室,河北保定071000

出　　处：《林业与生态科学》2025年第2期136-145,共10页Forestry and Ecological Sciences

基　　金：河北省自然科学基金(C2019204257);河北省高等学校科学技术研究项目青年基金(QN2019052);保定市揭榜挂帅项目(2022创332);保定市科技计划项目(2211G014)。

摘　　要：为探究饲料桑在盐碱逆境下的生理生化响应机制,以1 a生桂109号杂交饲料桑作为研究对象,采用盆栽法对饲料桑幼苗进行不同盐碱胁迫处理,分析饲料桑生理指标对不同盐碱胁迫响应的差异,探究其耐盐碱机理。结果表明,盐碱胁迫处理后,饲料桑幼苗的叶片形态受到抑制,且随着胁迫浓度的增加抑制加强。此外,其生理生化指标也表现出不同的变化趋势。随着盐碱浓度的增加和胁迫持续时间的延长,丙二醛含量呈上升趋势,其中T_(0.1%)和S_(0.2%)处理的叶片丙二醛含量整体呈现随时间出现先升后降的趋势,在胁迫第20天时达到峰值,之后显著下降;超氧化物歧化酶、过氧化物酶、过氧化氢酶、脯氨酸和可溶性蛋白含量整体呈现出先升后降的趋势,试验分析发现,与盐胁迫相比,在进行混合胁迫时,细胞膜质过氧化伤害更大。因此,饲料桑在盐碱胁迫下主要通过积累渗透调节物质、提高抗氧化酶系统活性来抵御盐碱胁迫,其中抗氧化酶系统均呈现“低促高抑”的现象。In order to explore the physiological and biochemical response mechanism of forage mulberry under salt-alkali stress,the 1-year Shenggui-109 hybrid forage mulberry was taken as the research object,and different salt-alkali stress treatments were carried out on forage mulberry seedlings by potted method,the difference of physiological indexes of forage mulberry to different salt-alkali stress treatments was analyzed,and the mechanism of salt-alkali tolerance was explored.The results showed that the leaf morphology of forage mulberry seedlings was inhibited after salt and alkali stress treatment,and the inhibition was strengthened with the increase of stress concentration.In addition,the physiological and biochemical indexes also showed different trends.Malondialdehyde content showed an increasing trend with the increase of salt and alkali concentration and the extension of stress duration.The malondialdehyde content of leaves treated with T_(0.1%)and S_(0.2%)showed a trend of first increasing and then decreasing with time,reaching a peak value on the 20th day of stress,and then decreased significantly.The contents of superoxide dismutase,peroxidase,catalase,proline and soluble protein increased first and then decreased.The experimental analysis showed that compared with salt stress,the membrane peroxidation damage was greater under mixed stress.Therefore,forage mulberry can resist salt-alkali stress mainly by accumulating osmotic regulatory substances and improving the activity of antioxidant enzyme system,among which the antioxidant enzyme system showed a phenomenon of"low promotion and high inhibition".

关 键 词：饲料桑 盐碱胁迫 生长状况 生理特性 耐盐机理 

分 类 号：S759.39[农业科学—森林经理学]