兼性内生巨大芽孢杆菌B6对杞柳转运铅、镉的影响  

Effect of facultative endophytic Bacillus megaterium B6 on transport of Pb and Cd by Salix integra

作　　者：陈平 周健 王倩[1] 苏蓓蓓 黄大庄[1] 牛小云 CHEN Ping;ZHOU Jian;WANG Qian;SU Beibei;HUANG Dazhuang;NIU Xiaoyun(College of Landscape Architecture and Tourism,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学园林与旅游学院,河北保定071000

出　　处：《林业与生态科学》2025年第2期146-154,共9页Forestry and Ecological Sciences

基　　金：国家自然科学基金项目(32301550);河北省自然科学基金项目(C2022204161);河北省教育厅青年基金项目(QN2022120)。

摘　　要：为了探究兼性内生巨大芽孢杆菌B6对杞柳转运重金属的作用机制,以水培生根的当年生杞柳为研究材料,通过盆栽试验,研究菌株B6不同的接种方式对杞柳转运Pb、Cd的影响及其作用机制。结果表明:(1)菌株B6显著促进了Pb的转运,抑制了Cd的转运。(2)杞柳根系与土壤同时接种巨大芽孢杆菌B6对Pb的转运作用高于仅在杞柳根系接种菌株B6,杞柳叶和枝部的促进效果分别提升了65.80%和42.74%。(3)结构方程分析表明,接种巨大芽孢杆菌B6直接促进Pb的转运,但对Cd的转运无直接作用。(4)接种菌株B6后速效氮、磷、钾含量显著增加,有机质含量降低;速效氮和速效钾显著促进土壤中重金属的生物有效性,速效磷和有机质则显著抑制土壤中重金属的生物有效性。最终土壤中重金属有效性通过影响杞柳地上部分重金属浓度间接促进Pb转运系数的提高。因此,菌株B6通过直接与间接作用共同调控重金属转运,且土壤与杞柳根系同时接种菌株B6对重金属转运的促进作用更大。In order to explore the mechanisms of Bacillus megaterium B6 on the transport of heavy metals in Salix integra,this study took the rooted annual S.integra in hydroponics as the research material,and conducted pot experiments to study the effects and mechanisms of different inoculation methods of B6 on the transport of Pb and Cd.The results showed that:(1)Strain B6 significantly promoted the transport of Pb and inhibited the transport of Cd.(2)The Pb transport ability was higher in the treatment of inoculation with B.megaterium B6 in root system and soil simultaneously than that inoculation with strain B6 in the root only,the promoting effect of leaves and branches of S.integra was increased by 65.80%and 42.74%respectively.(3)Structural equation modeling indicated that B.megaterium B6 promoted the transport coefficient of Pb directly,but it had no direct effect on the transport coefficient of Cd.(4)After inoculation with strain B6,the contents of available nitrogen,phosphorus and potassium increased significantly,while the content of organic matter decreased.The bioavailability of heavy metals in soil was significantly promoted by available nitrogen and available potassium,while significantly inhibited by available phosphorus and organic matter.Finally,the availability of heavy metals in soil indirectly promoted the improvement of Pb transport coefficient by affecting the concentration of heavy metals in the aboveground part of S.integra.Hence,strain B6 regulated heavy metal transport through direct and indirect effects,and the promotion effect of simultaneous inoculation of soil and S.integra root system on heavy metal transport was greater.

关 键 词：铅、镉污染 植物修复 巨大芽孢杆菌 转运系数 

分 类 号：X53[环境科学与工程—环境工程]