Peri-centrosomal localization of small interfering RNAs in C.elegans  

作　　者：Qile Jin Xuezhu Feng Minjie Hong Ke Wang Xiangyang Chen Jiewei Cheng Yan Kuang Xiaoyue Si Mingjing Xu Xinya Huang Shouhong Guang Chengming Zhu 

机构地区：[1]Department of Obstetrics and Gynecology,The First Affiliated Hospital of USTC,The USTC RNA Institute,Ministry of Education Key Laboratory for Membraneless Organelles&Cellular Dynamics,Hefei National Research Center for Physical Sciences at the Microscale,Center for Advanced Interdisciplinary Science and Biomedicine of IHM,School of Life Sciences,Division of Life Sciences and Medicine,Biomedical Sciences and Health Laboratory of Anhui Province,University of Science and Technology of China,Hefei 230027,China [2]School of Basic Medicine,Anhui Medical University,Hefei 230032,China

出　　处：《Science China(Life Sciences)》2025年第4期895-911,共17页中国科学(生命科学英文版)

基　　金：funded by the National Institutes of Health(NIH)Office of Research Infrastructure Programs(P40 OD010440);supported by grants from the National Natural Science Foundation of China(32230016 awarded to S.G.,32270583 awarded to C.Z.,32070619 awarded to X.F.,2023M733425 awarded to X.H.,and 32300438 awarded to X.H.);the National Key R&D Program of China(2022YFA1302700 to S.G.);the Research Funds of Center for Advanced Interdisciplinary Science and Biomedicine of IHM(QYPY20230021 awarded to S.G.);the Fundamental Research Funds for the Central Universities。

摘　　要：The centrosome is the microtubule-organizing center and a crucial part of cell division.Centrosomal RNAs(cnRNAs)have been reported to enable precise spatiotemporal control of gene expression during cell division in many species.Whether and how cnRNAs exist in C.elegans are unclear.Here,using the nuclear RNAi Argonaute protein NRDE-3 as a reporter,we observed potential peri-centrosome localized small interfering(si)RNAs in C.elegans.NRDE-3 was previously shown to associate with pre-mRNAs and pre-rRNAs via a process involving the presence of complementary siRNAs.We generated a GFP-NRDE-3 knock-in transgene through CRISPR/Cas9 technology and observed that NRDE-3 formed peri-centrosomal foci neighboring the tubulin protein TBB-2,other centriole proteins and pericentriolar material(PCM)components in C.elegans embryos.The peri-centrosomal accumulation of NRDE-3 depends on RNA-dependent RNA polymerase(RdRP)-synthesized 22G siRNAs and the PAZ domain of NRDE-3,which is essential for siRNA binding.Mutation of eri-1,ergo-1,or drh-3 significantly increased the percentage of pericentrosome-enriched NRDE-3.At the metaphase of the cell cycle,NRDE-3 was enriched in both the peri-centrosomal region and the spindle.Moreover,the integrity of centriole proteins and pericentriolar material(PCM)components is also required for the peri-centrosomal accumulation of NRDE-3.Therefore,we concluded that siRNAs could accumulate in the pericentrosomal region in C.elegans and suggested that the peri-centrosomal region may also be a platform for RNAi-mediated gene regulation.

关 键 词：SIRNA centrosomal RNAs NRDE-3 

分 类 号：Q75[生物学—分子生物学]