60Co-γ射线辐照对山桐子种子M_(1)代生长的刺激效应  

Growth-stimulating effect of 60Co-γray irradiation on Idesia polycarpa seeds in M_(1) generation

作　　者：许恒 赵雪花 郑婉露 李德贵 张丹丹 张子呈 李翔[3,4] XU Heng;ZHAO Xuehua;ZHENG Wanlu;LI Degui;ZHANG Dandan;ZHANG Zicheng;LI Xiang(Forestry Bureau of Liupanshui City,Liupanshui 553001,China;Helongjiang Research Institute of Atomic Energy,Harbin 150081,China;College of Forestry and Grassland,Ningxia University,Yinchuan 750021,China;National Key Laboratory of Efficient Production of Forest Tree Resources,Yinchuan 750021,China)

机构地区：[1]六盘水市林业局,六盘水553001 [2]黑龙江省原子能研究院,哈尔滨150081 [3]宁夏大学林业与草业学院,银川750021 [4]林木资源高效生产全国重点实验室,银川750021

出　　处：《辐射研究与辐射工艺学报》2025年第2期58-71,共14页Journal of Radiation Research and Radiation Processing

摘　　要：本研究以贵州六盘水市野生山桐子种子为实验材料,采用不同吸收剂量的60Co-γ射线对种子进行处理,调查60Co-γ射线辐照对山桐子幼苗生长过程中的成苗率及1年生山桐子的地径、苗高、叶长、叶宽、叶面积、叶周长、二级根长度、二级根粗度等16个生长指标及叶绿素含量的影响。结果表明,与对照相比,60Co-γ射线辐照处理显著增加了山桐子成苗率,并对山桐子幼苗生长具有显著影响(p<0.05),吸收剂量为120 Gy时,山桐子幼苗地径和苗高均最大,分别比对照增加了36%和68%;随着吸收剂量增加,二级根长度、二级根粗度和三级根数量均显著增加,在120 Gy时最大;山桐子叶片的叶绿素含量总体呈现上升趋势;60Co-γ射线辐照处理产生了高生长、茎秆弯曲、畸形、多分枝和矮化型等类型的突变植株;60Co-γ射线吸收剂量与大多数生长性状均存在显著相关性(p<0.05)。以上研究结果说明,60Co-γ射线辐照可以提高山桐子的出苗率,促进山桐子生长及根系发育,获得少量突变体植株,为山桐子新种质创制提供了重要的理论基础。The seeds of wild Idesia polycarpa obtained from Liupanshui City,Guizhou Province were used as experimental materials in this study,where the seeds were treated with different irradiation doses of 60Co-γray.The effect of 60Co-γray irradiation on the seedling rate,ground diameter,seedling height,leaf length,leaf width,leaf area,leaf circumference,secondary root length,secondary root diameter,and chlorophyll content of 1-year-old I.polycarpa were investigated.The results showed that compared with the control,60Co-γirradiation treatment significantly increased the seedling rate of I.polycarpa and had significant effects on the growth of I.polycarpa seedlings(p<0.05).Under an irradiation dose of 120 Gy,the ground diameter and seedling height of the I.polycarpa seedlings were the largest,i.e.,36%and 68%higher than those of the control,respectively.With the increase in irradiation dose,the length of secondary roots,the thickness of secondary roots,and the number of tertiary roots increased significantly,reaching maximum values at 120 Gy.The chlorophyll content of I.polycarpa leaves first increased,then decreased,and then increased again.Mutant plants with high growth,stem bending,deformity,multibranching,and dwarfing were produced via 60Co-γray irradiation.The irradiation dose of 60Co-γray was significantly correlated with most of the growth traits(p<0.05).The results above show that 60Co-γray irradiation can improve the seedling emergence rate of I.polycarpa,promote the growth and root development of I.polycarpa,and yield a few different types of mutant plants,thus providing an important theoretical basis for the creation of new germplasms of I.polycarpa.

关 键 词：山桐子 60Co-γ 射线 出苗率 幼苗生长 叶绿素 

分 类 号：TL99[核科学技术—核技术及应用]