梓醇调控涎腺上皮细胞lnc-NONHSAT071210表达治疗干燥综合征模型小鼠的作用机制  

Catalpol regulates expression of lnc-NONHSAT071210 in salivary gland epithelial cells for therapy mechanism of Sjögren's syndrome model mice

作　　者：何伟倩 吴春凤 李小芬[1] 刘媛 郭予洁[3] HE Weiqian;WU Chunfeng;LI Xiaofen;LIU Yuan;GUO Yujie(Department of Rheumatology,Liuzhou People's Hospital,Liuzhou 545200,China;Department of Laboratory,Liuzhou People's Hospital,Liuzhou 545200,China;Cardiovascular Medicine,Liuzhou People's Hospital,Liuzhou 545200,China)

机构地区：[1]柳州市人民医院风湿免疫科,柳州545200 [2]柳州市人民医院检验科,柳州545200 [3]柳州市人民医院心血管内科,柳州545200

出　　处：《中国免疫学杂志》2025年第4期798-802,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金(82060303);柳州市科技计划项目(2020NBAA0804)。

摘　　要：目的:观察梓醇对干燥综合征(SS)模型小鼠的治疗作用,并通过体外实验探讨其作用机制。方法:8周龄NOD小鼠给予100 mg/kg梓醇灌胃8周,检测血清中IFN-γ和IL-17表达,观察小鼠唾液腺病理;50μmol/L梓醇处理lnc-NONHSAT071210 shRNA转染的唾液腺上皮细胞系,检测lnc-NONHSAT071210表达;再以10 ng/ml IFN-γ干预细胞,lnc-NONHSAT071210 shRNA及梓醇处理72 h,检测细胞增殖及IL-17和IFN-γ表达。结果:与对照组相比,梓醇组小鼠血清中IFN-γ及IL-17表达下降(P<0.05),唾液腺病理淋巴细胞浸润减少,腺体结构破坏减轻;与IFN-γ干预组相比,梓醇处理后唾液腺上皮细胞增殖水平升高,IFN-γ及IL-17表达下降(P<0.05);梓醇处理后,lnc-NONHSAT071210表达下降(P<0.05),且梓醇及lnc-NONHSAT071210 shRNA共同干预后,IFN-γ及IL-17表达下降更为显著(P<0.01)。结论:梓醇可抑制SS模型小鼠血清炎症因子表达和唾液腺淋巴细胞浸润程度,通过调节lnc-NONHSAT071210抑制涎腺导管上皮细炎症反应,抑制SS进展。Objective:To observe therapeutic effect of catalpol on Sjögren's syndrome(SS)model mice and explore further mechanism through vitro experiments.Methods:Eight-week-old NOD mice were given catalpol 100 mg/kg by gavage for 8 weeks.Serum levels of IFN-γand IL-17 were measured.Pathological of salivary glands were observed.lnc-NONHSAT071210 shRNA transfected salivary gland epithelial cell lines were treated with 50μmol/L catalpol,and expression of lnc-NONHSAT071210 was detected.Cells were intervented by 10 ng/ml IFN-γ,lnc-NONHSAT071210 shRNA and catalpol treatment for 72 h,cell proliferation and expressions of IL-17 and IFN-γwere detected.Results:Compared with control group,expressions of IFN-γand IL-17 were decreased(P<0.05),pathology of salivary gland showed that infiltration of lymphocytes was reduced and destruction of gland structure was significantly reduced in catalpol group.Compared with IFN-γintervention group,catalpol treatment significantly increased proliferation of salivary gland epithelial cells,and decreased expressions of IFN-γand IL-17(P<0.05).After catalpol treatment,expression of lnc-NONHSAT071210 was decreased(P<0.05).Moreover,IFN-γand IL-17 expressions were decreased by catalpol and lnc-NONHSAT071210 shRNA co-treatment(P<0.01).Conclusion:Catalpol can inhibit expressions of inflammatory cytokines in serum and infiltration of lymphocyte in salivary gland of SS model mice,and inhibit salivary gland ductal fine epithelial inflammatory response and progression of SS by regulating lnc-NONHSAT071210.

关 键 词：NOD小鼠 梓醇 干燥综合征 涎腺上皮细胞 长链非编码RNA 

分 类 号：R593[医药卫生—内科学]