黄芩素调控TLR4/NF-κB通路对慢性阻塞性肺疾病大鼠气道炎症的影响  

Impact of baicalein on airway inflammation in COPD rats by regulating TLR4/NF-κB pathway

作　　者：张林 徐厚平 刘代群 汪思嘉 李光霞 ZHANG Lin;XU Houping;LIU Daiqun;WANG Sijia;LI Guangxia(General Medicine Department of Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University,Luzhou 646000,China;Preventive Medicine Center of Traditional Chinese Medicine Hospital Affiliated to Southwest Medical University,Luzhou 646000,China)

机构地区：[1]西南医科大学附属中医医院全科医学科,泸州646000 [2]西南医科大学附属中医医院治未病中心,泸州646000

出　　处：《中国免疫学杂志》2025年第4期903-907,共5页Chinese Journal of Immunology

摘　　要：目的:探讨黄芩素(BA)调控TLR4/NF-κB通路对慢性阻塞性肺疾病(COPD)大鼠气道炎症的影响。方法:大鼠随机分为造模组(56只)及假手术组(10只),造模组大鼠通过烟熏及气管滴注LPS建立COPD模型,造模成功大鼠随机分为COPD组、BA低剂量(BA-L,100 mg/kg)组、BA高剂量(BA-H,200 mg/kg)组、TLR4激活剂LPS组(0.1 mg/kg)、BA-H+LPS组(0.1 mg/kg LPS+200 mg/kg BA)。干预结束2 h后检测大鼠呼气峰流速(PEF)、1秒用力呼气体积(FEV1)、用力肺活量(FVC)等肺功能指标;检测大鼠血清TNF-α、IL-6、IL-8水平;收集支气管肺泡灌洗液(BALF)分析细胞数目及分类;分离肺组织分析其病理学变化及TLR4、p-NF-κB p65/NF-κB p65表达。结果:与假手术组相比,COPD组病理损伤严重,PEF、FEV1、FVC显著降低,血清IL-6、IL-8、TNF-α水平、白细胞数、中性粒细胞、淋巴细胞比例、TLR4、p-NF-κB p65/NF-κB p65表达显著升高(P<0.05);与COPD组相比,LPS组病理损伤进一步加重,PEF、FEV1、FVC显著降低,血清IL-6、IL-8、TNF-α水平、白细胞数、中性粒细胞、淋巴细胞比例、TLR4、p-NF-κB p65/NF-κB p65表达显著升高(P<0.05),BA-L组、BA-H组病理损伤逐渐改善,PEF、FEV1、FVC显著升高,血清IL-6、IL-8、TNF-α水平、白细胞数、中性粒细胞、淋巴细胞比例、TLR4、p-NF-κB p65/NF-κB p65表达显著降低(P<0.05);LPS逆转了BA-H对COPD大鼠的保护作用。结论:BA可改善COPD大鼠气道炎症反应,可能与抑制TLR4/NF-κB通路有关。Objective:To investigate impact of baicalein(BA)on airway inflammation in chronic obstructive pulmonary disease(COPD)rats by regulating TLR4/NF-κB pathway.Methods:Rats were randomly divided into model group(n=56)and sham operation group(n=10),COPD rat model was established by smoking and intratracheal instillation of LPS,successful modeling rats were randomly divided into COPD group,BA low dose(BA-L,100 mg/kg)group,BA high dose(BA-H,200 mg/kg)group,TLR4 activator LPS group(0.1 mg/kg)and BA-H+LPS group(0.1 mg/kg LPS+200 mg/kg BA).After 2 h,peak expiratory flow(PEF),forced expiratory volume in first second(FEV1),forced vital capacity(FVC)and other lung function indexes were measured;serum TNF-α,IL-6 and IL-8 levels were measured;bronchoalveolar lavage fluid(BALF)was collected to analyze number and classification of cells;lung tissues were isolated to analyze pathological changes and TLR4,p-NF-κB p65/NF-κB p65 expressions.Results:Compared with sham operation group,pathological damage in COPD group was serious,PEF,FEV1 and FVC were decreased significantly,serum IL-6,IL-8,TNF-αlevels,leukocytes number,neutrophils and lymphocytes proportions,TLR4 and p-NF-κB p65/NF-κB p65 expressions were increased significantly(P<0.05);compared with COPD group,pathological damage in LPS group was further aggravated,PEF,FEV1 and FVC were reduced significantly,serum IL-6,IL-8,TNF-αlevels,leukocytes number,neutrophils and lymphocytes proportions,TLR4 and p-NF-κB p65/NF-κB p65 expressions were increased significantly(P<0.05),pathological damage in BA-L group and BA-H group gradually improved,PEF,FEV1 and FVC were increased significantly,serum IL-6,IL-8,TNF-αlevels,leukocytes number,neutrophils and lymphocytes proportions,TLR4 and p-NF-κB p65/NF-κB p65 expressions were decreased significantly(P<0.05);LPS reversed protective effect of BA-H on COPD rats.Conclusion:BA can improve airway inflammation in COPD rats,which may be related to inhibition of TLR4/NF-κB pathway.

关 键 词：黄芩素 TLR4/NF-κB通路 气道炎症 慢性阻塞性肺疾病 

分 类 号：R285.5[医药卫生—中药学]