不同季节甲醛消毒对罗非鱼鱼卵菌群的影响  

Effects of formaldehyde disinfection on the surface symbiotic microbiota of tilapia eggs in different seasons

作　　者：王佳文 关博元 蔡岩[1,2] 李美静 赵章定 王世锋 WANG Jiawen;GUAN Boyuan;CAI Yan;LI Meijing;ZHAO Zhangding;WANG Shifeng(School of Marine Biology and Fisheries,Hainan University,Haikou 570228,China;Key Laboratory of Tropical Hydrobiology and Biotechnology of Hainan Province,Haikou 570228,China;Hainan Baolu Aquatic Products Technology Co.,Ltd,Haikou 571822,China)

机构地区：[1]海南大学海洋生物与水产学院,海南海口570228 [2]海南省热带水生生物技术重点实验室,海南海口570228 [3]海南宝路水产科技有限公司,海南海口571822

出　　处：《海南大学学报(自然科学版中英文)》2025年第1期15-24,共10页Natural Science of Hainan University

基　　金：海南省重点研发项目(ZDYF2021XDNY276);横向项目(HD-KYH-2022002)。

摘　　要：为研究罗非鱼人工孵化流程中甲醛消毒对鱼卵表面共生菌群的影响,利用16S rRNA高通量测序技术对罗非鱼亲鱼塘未消毒鱼卵(消毒前鱼卵,EP)以及经甲醛消毒后放入孵化系统第2 d的鱼卵(消毒后鱼卵,E2)的表面共生菌群进行分析和功能预测。结果显示,在春季、秋季和冬季,EP鱼卵OTU数目高于E2鱼卵,EP鱼卵Ace、Chao1指数显著高于E2鱼卵(P<0.05),EP鱼卵Simpson指数则显著低于E2鱼卵(P<0.05);消毒前后微生物群落结构PcoA分析显示4个季节均存在显著性差异(P<0.05);在门水平上,E2鱼卵上拟杆菌门丰度比EP鱼卵增加,在秋季更显著(P<0.05);在属水平上,E2鱼卵上鲸杆菌属、芽孢杆菌属和弧菌属丰度显著低于EP鱼卵(P<0.05),而黄杆菌属、假单胞菌属和金黄杆菌属丰度显著增加(P<0.05)。菌群功能预测表明,氨基酸转运和新陈代谢、无机离子运输和代谢、碳水化合物运输和代谢为主要的预测功能。以上结果表明,罗非鱼鱼卵经甲醛消毒转移至室内孵化系统2 d后,其表面共生菌群结构发生显著变化且物种多样性显著降低;潜在益生菌丰度降低,潜在致病菌丰度升高;微生物功能特征无显著差异。In order to investigate the effects of formaldehyde disinfection on the symbiotic bacterial community on the surface of the tilapia eggs during artificial incubation,in the report,16S rRNA high-throughput sequencing technology was used to analyze and predict the surface symbiotic bacterial community of the unsterilized tilapia eggs(pre disinfection eggs,EP)collected from tilapia parent fish pond and the eggs(post disinfection eggs,E2)placed in the incubation system on the second day after formaldehyde disinfection.The results indicated that during spring,autumn,and winter,the number of OTUs in EP fish eggs were higher than that in E2 fish eggs,and the Ace and Chao1 indices of EP fish eggs were significantly higher than those of E2 fish eggs(P<0.05),the Simpson index of EP fish eggs were significantly lower than that of E2 fish eggs(P<0.05).The PcoA analysis results indicated that before and after disinfection,there were significant differences between the microbial community structure in all four seasons(P<0.05).At the phylum level,compared to EP fish eggs,the abundance of Bacteroidetes on E2 fish eggs increased,and which was more significant in autumn(P<0.05).At the genus level,the abundance of Cetobacterium,Bacillus,and Vibrio genera on E2 fish eggs were significantly lower than that on EP fish eggs(P<0.05),while the abundance of Flavobacterium,Pseudomonas,and Chryseobacterium significantly increased(P<0.05).The prediction of microbial community functions showed that amino acid transport and metabolism,inorganic ion transport and metabolism,and carbohydrate transport,and metabolism were the main predictive functions.After being disinfected with formaldehyde and transferred to an indoor incubation system for 2 days,the surface symbiotic bacterial community structure of tilapia eggs underwent significant changes and the species diversity significantly decreased.The abundance of potential probiotics decreased.There were no significant differences in the microbial functional characteristics.Our finding provided the

关 键 词：罗非鱼鱼卵 消毒 孵化系统 16S rRNA 微生物群落组成 

分 类 号：S949[农业科学—水产养殖]