聚乳酸-羟基乙酸支架复合骨髓间充质干细胞移植对大鼠创伤性脑损伤的影响  

The Effect of Polylactic Acid-Glycolic Acid Scaffold Combined with Transplantation of Bone Marrow Mesenchymal Stem Cells on Traumatic Brain Injury in Rats

作　　者：蒋晓倩 林恒 刘一鸣 李文英 谢冰清 杨朝鲜 JIANG Xiao-qjan;LIN Heng;LIU Yi-ming;LI Wen-ying;XIE Bing-qing;YANG Chao-xian(Suining Central Hospital Rehabilitation Center,Suining 629000,China;Department of Anatomy,School of Basic Medicine,Southwest Medical University,Luzhou 646000,China;Laboratory of Neurological Diseases and Brain Function,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,China;Department of Pediatrics,Southwest Medical University,Luzhou 646000,China)

机构地区：[1]遂宁市中心医院康复中心,遂宁629000 [2]西南医科大学基础医学院解剖教研室,泸州646000 [3]西南医科大学附属医院神经疾病与脑功能研究室,泸州646000 [4]西南医科大学儿科系,泸州646000

出　　处：《四川解剖学杂志》2024年第5期1-5,30,共6页Sichuan Journal of Anatomy

基　　金：沪州市应用基础研究项目(2021-JYJ-75);四川省自然科学基金(2022NSFSC0718);四川省科技计划(2022YFS0615)资助。

摘　　要：目的:探讨高分子材料聚乳酸-羟基乙酸(PLGA)复合骨髓间充质干细胞(BMSCs)移植对脑创伤灶的修复作用.方法:取大鼠骨髓进行BMSCs分离、纯化,观察BMSCs的神经诱导分化能力和在PLGA上的生长情况.用成年SD大鼠建立脑创伤模型并将其分为PLGA组、PLGA-BMSCs组、对照组和假手术组.前两组分别移植PLGA和PLGA-BMSCs进行治疗.各组术后 14d进行DAPI和免疫荧光染色观察脑组织结构、创伤处细胞迁移及突触相关蛋白的表达情况.结果:培养的BMSCs能诱导为神经元样细胞且能在PLGA支架上生长;PLGA及PLGA-BMSCs移植均能修复受损的脑组织;与PLGA组相比较,PLGA-BMSCs组的微管相关蛋白(MAP)2、突触后致密区蛋白(PSD)95 和突触素表达升高,而胶质纤维酸性蛋白(GFAP)表达降低.结论:PLGA-BMSCs比单纯PLGA移植能更好地修复脑创伤后的组织缺损.Objective:To investigate the repairing efect of polylactic acid-glycolic acid(PLGA)combined with bone marrow mesenchymal stem cells(BMSCs)transplantation on brain traumafoci.Methods:The BMSCs were isolated and purified from rat bone marrow.The neural differentiation ability of BMSCs and the growth of BMSCs on PLGA were observed.Adult SD rats were used and divided into PLGA,PLGA-BMSCs,control and sham groups.The PLGA and PLGA-BMSCs groups were established for the models of traumatic brain injury(TBI)and were transplanted with PLGA and PLGA-BMSCs,respectively.DAPI and immunofluorescence staining were performed on rats in each group 14 days after operation to observe brain tissue structure,cell migration and synaptic related protein expression.Results:The cultured BMSCs were induced into neuron-like cells and grew well on PLGA scaffolds.Both PLGA and PLGA-BMSCs could repair damaged brain tissue.The expressions of microtubule-associated protein(MAP)2,postsynaptic density protein(PSD)95 and synaptophysin increased,while the expression of glial fibrillary acidic protein(GFAP)decreased in the PLGA-BMSCs group,comparing with the PLGA group.Conclusion:PLGA scaffold combined with BMSCs is more effective than PLGA scaffold alone in repairing tissue defects in rats after brain trauma.

关 键 词：创伤性脑损伤 聚乳酸-羟基乙酸 骨髓间充质干细胞 大鼠 

分 类 号：R651.1[医药卫生—外科学]