Liposome of a synergistic combination of HDP-mimicking polymer and rifampicin for treating bacterial infections in aquaculture via oral administration  

基于HDP模拟聚合物与利福平协同组合的口服脂质体治疗水产养殖细菌感染

作　　者：Haowen Yu Luhang Chen Yueming Wu Wenhui Dai Minzhang Chen Yuan Chen Longqiang Liu Jiangzhou Wang Xinqi Huang Kang Chen Runhui Liu 于昊文;陈鲁航;武月铭;代文慧;陈敏璋;陈袁;刘龙强;汪江舟;黄欣琦;陈康;刘润辉

机构地区：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China [2]Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism,Key Laboratory for Ultrafine Materials of Ministry of Education,Frontiers Science Center for Materiobiology and Dynamic Chemistry,Engineering Research Center for Biomedical Materials of Ministry of Education,School of Materials Science and Engineering,East China University of Science and Technology,Shanghai 200237,China [3]Department of Biomaterials and Stem Cells,Suzhou Institute of Biomedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,China

出　　处：《Science China Materials》2025年第4期1259-1270,共12页中国科学(材料科学)(英文版)

基　　金：supported by the National Natural Science Foundation of China(T2325010,52203162,22075078);the National Key Research and Development Program of China(2022YFC2303100);the Fundamental Research Funds for the Central Universities(JKVD1241029,JKD01241701);the Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism(Shanghai Municipal Education Commission)。

摘　　要：The extensive use of antibiotics in high-density aquaculture has accelerated the emergence of antibiotic-resistant bacteria.The presence of antibiotics in water and soil,and their transmission through the food chain exacerbate the pressures associated with antibiotic resistance,posing a significant threat to human health.Despite this challenge and serious issue,effective alternatives to antibiotics remain lacking.In this study,we report a potent synergistic effect between an HDP-mimicking polymer,DLLys90-BLG10,and rifampicin against Vibrio species.This synergistic combination substantially reduces the required dosage of rifampicin,thereby mitigating the risk of antibiotic resistance development and the release of rifampicin into water and soil.Furthermore,we developed a cell-penetrating peptide-modified liposome system that can co-encapsulate DLLys90-BLG10 and rifampicin,which exerts potent antibacterial activity against Vibrio both in vitro and in vivo,while maintaining bio-compatibility.Additionally,oral administration of CPP-Lip@Syn showed effective control of Vibrio infections.This strategy opens new avenues in exploring antibiotic alternatives in aquaculture and implies potential applications in aquaculture.在高密度水产养殖中,大量使用抗生素正加速耐药菌的出现.水体与土壤中的抗生素残留以及其经食物链的传播,使耐药性问题日益严峻,并对人类健康构成重大威胁.尽管这一问题尤为紧迫,但目前仍缺乏有效的抗生素替代策略.在本研究中,我们发现一种宿主防御肽(HDP)模拟聚合物DLLys90-BLG10与利福平之间存在显著的协同增效作用.该协同组合可大幅降低利福平用量,从而有效减缓耐药性的产生及利福平向水体和土壤的释放.进一步,我们构建了一种经细胞穿透肽修饰的脂质体系统,将DLLys90-BLG10与利福平共同包封,实现了在体外与体内条件下对弧菌的高效抑菌效果,同时维持良好的生物相容性.此策略为探索水产养殖中抗生素替代品开辟了新思路,并有望在该领域发挥潜在应用价值.

关 键 词：liposomes oral administration AQUACULTURE synergistic combinations Vibrio infections 

分 类 号：S94[农业科学—水产养殖]