草莓细菌性冠腐病的病原菌分离鉴定与防治研究  

Pathogen identification and biological control of strawberry bacterial crown rot

作　　者：翟婧 彭一文 郑永利 寿林飞 吴慧明[3] 吴降星 许燎原 郑振浩 冯晓晓 Zhai Jing;Peng Yiwen;Zheng Yongli;Shou Linfei;Wu Huiming;Wu Jiangxing;Xu Liaoyuan;Zheng Zhenhao;Feng Xiaoxiao(Agricultural Technology Extension Station of Ning bo,Ningbo Zhejiang 315012,China;Zhejiang Agricultural Products Green Development Center,Hangzhou Zhejiang 310003,China;College of Advanced Agriculture Sciences,Zhejiang Agricultural and Forestry University,Hangzhou Zhejiang 311300,China;Zhejiang Master Station of Plant Protection Quarantine and Pesticide Management,Hangzhou Zhejiang 310000,China;The Rural Development Academy and Agricultural Experiment Station,Zhejiang University,Hangzhou Zhejiang 310058,China)

机构地区：[1]浙江省宁波市农业技术推广总站,浙江宁波315012 [2]浙江省农产品绿色发展中心,浙江杭州310003 [3]浙江农林大学现代农学院,浙江杭州311300 [4]浙江省植保检疫与农药管理总站,浙江杭州310000 [5]浙江大学新农村发展研究院与农业试验站,浙江杭州310058

出　　处：《中国植保导刊》2025年第2期16-21,共6页China Plant Protection

基　　金：宁波市农业技术推广项目(2021NT015);浙江省“三农九方”科技协作计划(2022SNJF015);浙江省教育厅一般科研项目(Y2022-49712);宁波市科学技术局公益类科技计划重点项目(2021S022)。

摘　　要：草莓细菌性冠腐病是近年来国内草莓生产上新暴发的一种细菌性病害。以浙江省杭州市萧山区、建德市,宁波市慈溪市,嘉兴市嘉善县和湖州市德清县设施栽培中的发病草莓为材料,分离并鉴定致病菌,并开展田间试验,探究5种生物菌剂的防病效果。经形态观察、革兰氏染色、全基因组测序及系统发育树分析,明确采集地草莓细菌性冠腐病的病原为荧光假单胞菌群。田间试验结果表明,5种供试药剂中,贝莱斯芽孢杆菌ZN-S10对该病的防效最好,通过移栽时蘸根、移栽后灌根的施药措施,可有效降低草莓的死株率,防治效果接近70%。本研究结果可为科学预防和控制该病提供依据。Strawberry bacterial crown rot has emerged as a novel diseasein the strawberry production sector in China in recent years. In this study, infected plants were collected from various regions within Zhejiang Province, including Xiaoshan District and Jiande City of Hangzhou, Cixi City of Ningbo, Jiashan County of Jiaxing, and Deqing County of Huzhou. These samples were used as test objects to identify the pathogenic bacteria. Concurrently, field trials were carried out to evaluate the efficacy of four biological agents in managing this disease. The results of these trials, in accordance with Koch's postulation, identified Pseudomonas spp. as the pathogen responsible for strawberry bacterial crown rot.Further identification through morphological observation, Gram staining, whole genome sequencing, and phylogenetic analysis determined this strain to be Pseudomonas fluorescens. The field trials showed that Bacillus velezensis ZN-S10 WP exhibited the most effective control of strawberry bacterial crown rot. Following the implementation of dipping and irrigation treatments of the root, the infection rate of strawberry could be effectively reduced, with a control effect approaching 70%. The results of this study can provide a foundation for the prevention and management of the disease.

关 键 词：草莓细菌性冠腐病 假单胞菌 病原鉴定 生物菌剂 防控 

分 类 号：S436.639[农业科学—农业昆虫与害虫防治]