扬州市医疗机构口腔诊疗用水污染情况分析及两种培养基检测效能比较  

Analysis of contamination status in oral water for diagnosis and treatment of medical institutions in Yangzhou city and comparison of detection efficacy between two culture media

作　　者：许蓉蓉 姜天佑 朱倩 田婧棽 陆荣荣 周乐 许培 周信 XU Rong-rong;JIANG Tian-you;ZHU Qian;TIAN Jing-shen;LU Rong-rong;ZHOU Le;XU Pei;ZHOU Xin(Yangzhou City Center for Disease Control and Prevention,Yangzhou,Jiangsu 225007,China;Hanjiang District Center for Disease Control and Prevention,Yangzhou City)

机构地区：[1]扬州市疾病预防控制中心,江苏扬州225007 [2]扬州市邗江区疾病预防控制中心

出　　处：《预防医学论坛》2025年第1期31-34,共4页Preventive Medicine Tribune

基　　金：江苏省扬州市科技计划软课题研究项目(YZ2023314);江苏省扬州市卫生健康委员会科研项目(2023-2-33)。

摘　　要：目的了解江苏省扬州市医疗机构口腔诊疗用水的污染情况,比较两种培养方法对口腔诊疗用水细菌培养结果的影响,为口腔诊疗用水微生物培养方法的选择提供参考依据。方法2024年随机抽取江苏省扬州市8家市直直管综合医院、8家口腔诊所,共采集175份口腔诊疗用水,分别采用营养琼脂(NA)在37℃、48h条件下和R2琼脂(R2A)在20℃、168h条件下进行平行培养,分析口腔诊疗用水样本总体合格率,比较NA和R2A的菌落计数结果。结果口腔诊疗用水样本总体合格率为54.3%(95/175),其中综合医院样本合格率为61.2%(71/116),口腔诊所样本合格率为40.7%(24/59)。菌落计数结果显示,NA和R2A培养基中位数分别为63CFU/mL和1100CFU/mL,两者比较差异有统计学意义(Z=-10.316,P<0.001);Kappa一致性比较显示两种培养基结果判读一致性中等(Kappa=0.535)。结论医疗机构口腔诊疗用水具有潜在的污染风险,口腔诊所的口腔诊疗用水合格率低于综合医院。采用R2A培养基(20℃,168h)较NA培养基(37℃,48h)可以更灵敏地反映口腔诊疗用水的微生物污染情况。Objective To understand the pollution of oral water for diagnosis and treatment in medical institutions in Yangzhou city,Jiangsu province,and compare the influence of two culture methods on the bacterial culture results of oral water for diagnosis and treatment,so as to provide reference for the selection of oral water microbial culture methods.Methods In 2024,175water samples were randomly collected from 8municipal general hospitals and 8dental clinics in Yangzhou city.Parallel cultures were performed using Nutrient Agar(NA)at 37℃for 48hours and R2AAgar(R2A)at 20℃for 168hours.The overall qualification rate of oral water for diagnosis and treatment was analyzed,and the colony counts between NA and R2A were compared.Results The overall qualification rate of oral water for diagnosis and treatment was 54.3%(95/175),with a higher rate in general hospitals(61.2%,71/116)compared to dental clinics(40.7%,24/59).The median colony counts were 63CFU/mL for NA and 1100CFU/mL for R2A,showing a statistically significant difference(Z=-10.316,P<0.001).The Kappa consistency test indicated moderate agreement between the two culture methods(Kappa=0.535).Conclusion The oral diagnosis and treatment water in medical institutions has potential pollution risks,and the sample qualification rate of dental clinics is lower than that of general hospitals.Using R2Amedium(20℃,168h)can more sensitively reflect the microbial contamination of oral diagnostic and therapeutic water compared to NA medium(37℃,48h).

关 键 词：口腔诊疗用水 营养琼脂 R2琼脂 细菌 培养基 

分 类 号：R197.39[医药卫生—卫生事业管理] R118[医药卫生—公共卫生与预防医学]