全二维气相色谱-质谱顶空分析总状土木香精油成分  

Rapid Analysis of the Components of Essential Oil of Inula racemosa Hook.f.in the Headspace of the Comprehensive Two-dimensional Temperament

作　　者：严志宏[1] 许金娣 张臣 顿珠次仁 张宁方 罗珍 YAN Zhihong;XU Jindi;ZHANG Cheng;DUNZHU Ciren;ZHANG Ningfang;LUO Zhen(Jiangxi University of Chinese Medicine,Nanchang 330004,China;University of Tibetan Medicine,Lhasa 850007,China)

机构地区：[1]江西中医药大学,江西南昌330004 [2]西藏藏医药大学,西藏拉萨850007

出　　处：《林产化学与工业》2025年第2期1-9,共9页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金资助项目(82160827);2021年西藏藏医药大学中药学(藏药)博士点培育科研支撑计划项目(BSDPY-21-08);2024藏药博士点建设学科建设项目(BSDJS-XKJS-24-04)。

摘　　要：以产自四川的总状土木香为原料,采用水蒸气蒸馏法进行总状土木香精油的提取,建立总状土木香精油的全二维气相色谱-质谱联用(GC×GC-MS)分析方法,并以顶空进样法进行挥发性成分的GC×GC-MS分析,在单因素试验基础上,通过响应面法优化得到精油提取的最佳工艺条件为:粒径0.850 mm、浸泡时间6 h、提取时间10 h、液料比10∶1(mL∶g),该条件下精油得率为1.02%。利用GC×GC-MS对2种不同提取方式下的总状土木香挥发性成分进行分析,水蒸气蒸馏法共鉴定30个成分,而顶空进样共鉴定82个成分,共有成分8个,均主要以倍半萜类为主。而所得的总状土木香挥发性成分和含量有所差异,水蒸气蒸馏鉴定的主要精油成分和GC含量分别为土木香内酯29.65%、β-榄香烯28.11%、β-瑟林烯12.88%,顶空进样鉴定的主要挥发性成分和GC含量分别为土木香内酯46.34%和异土木香内酯41.17%。The essential oil of Inula racemosa Hook.f.from Sichuan Province was extracted by steam distillation.A comprehensive two-dimensional gas chromatography-mass spectrometry(GC×GC-MS)analysis method of essential oil of I.racemosa was established,and the GC×GC-MS analysis of volatile components was carried out by headspace sampling method.Based on single-factor experiments,the optimized process conditions of essential oil extraction were as follows:particle size of 0.850 mm,soaking time of 6 h,extraction time of 10 h,liquid-to-material ratio of 10∶1(mL∶g),with an essential oil yield of 1.02%under these conditions.GC×GC-MS was used to analyze the volatile components of I.racemosa under two different extraction methods.A total of 30 components were identified by steam distillation,while 82 components were identified by headspace sampling,with 8 common components,mainly sesquiterpenes.The volatile components and relative contents obtained from I.racemosa showed some differences.The main essential oil components and GC contents identified by steam distillation were 29.65%of alantolactone,at 28.11%ofβ-elemene,and 12.88%ofβ-selinene,respectively.In contrast,the main volatile components and relative contents identified by headspace sampling were at 46.34%of alantolactone and 41.17%of isoalantolactone,respectively.The optimized essential oil extraction process and headspace sampling method could be combined to obtain a more comprehensive volatile substances of I.racemosa.Utilizing GC×GC-MS technology for component analysis could serve as a rapid identification method for evaluating the quality of I.racemosa essential oil.

关 键 词：总状土木香 精油 全二维气相色谱-质谱技术 化学成分 

分 类 号：TQ35[化学工程]