数字图像比色法测定肉桂油中醛类化合物的研究  

Detection of Aldehydes in Cinnamon Oil by Digital Image Colorimetry Method

作　　者：程贤[1] 李胜男 李盼盼 陈玉湘[1] 李鹏飞[2] 毕良武[1] CHENG Xian;LI Shengnan;LI Panpan;CHEN Yuxiang;LI Pengfei;BI Liangwu(Institute of Chemical Industry of Forest Products,CAF,Key Lab.of Biomass Energy and Material,Jiangsu Province,Key Lab.of Chemical Engineering of Forest Products,National Forestry and Grassland Administration,National Engineering Research Center of Low-Carbon Processing and Utilization of Forest Biomass,Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources,Nanjing 210042,China;Key Laboratory of Chemistry and Engineering of Forest Products,State Ethnic Affairs Commission,Guangxi Key Laboratory of Chemistry and Engineering of Forest Products/Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products,Guangxi Minzu University,Nanning 530006,China)

机构地区：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,国家林业和草原局林产化学工程重点实验室,林木生物质低碳高效利用国家工程研究中心,江苏省林业资源高效加工利用协同创新中心,江苏南京210042 [2]广西民族大学化工学院,林产化学与工程国家民委重点实验室,广西林产化学与工程重点实验室/协同创新中心,广西南宁530006

出　　处：《林产化学与工业》2025年第2期19-25,共7页Chemistry and Industry of Forest Products

基　　金：国家自然科学基金青年项目(32201512);广西林产化学与工程重点实验室开放基金课题(GXFK2407)。

摘　　要：基于醛类化合物可与2,4-二硝基苯肼(DNPH)反应生成红色显色物质的原理,采集有颜色部位的图像,利用三基色红绿蓝(RGB)模式提取数据,建立了一种检测醛类化合物的数字图像比色法,用于检测肉桂油样品中醛类化合物,并对检测方法进行了条件优化和方法学验证。研究结果表明:针对20μL待测样品,加入1 mL的DNPH(5 mmol/L)、 10μL盐酸和1.5 mL氢氧化钾(60 g/L)完成显色反应,可利用样本颜色的蓝/绿(B/G)值作为指标建立肉桂醛测量方法,该方法标准曲线的相关系数为0.998 6,定量限为1 mg/L,检测限为0.5 mg/L,基质干扰为6.5%,日内和日间精密度分别为0.39%和0.52%,准确度为102.5%~107.5%,相对标准偏差(RSD)均小于6.5%。Based on the principle that aldehydes could react with 2,4-dinitrophenylhydrazine(DNPH)to produce red chromogenic substances,the images of colored parts were collected and the three-color red,green and blue(RGB)mode was used to obtain data.A digital image colorimetry method was established to detect aldehydes and applied to cinnamon oil samples,and the conditions of detection method were optimized and the methodology was verified.The results showed that for 20μL samples to be tested,1 mL DNPH(5 mmol/L),10μL hydrochloric acid and 1.5 mL potassium hydroxide(60 g/L)were added to complete the color reaction,and the cinnamaldehyde measurement method could be established using the blue/green(B/G)value of the sample color as an indicator.The correlation coefficient of the standard curve of the method was 0.9986,the limit of quantitation was 1 mg/L,the detection limit was 0.5 mg/L,the matrix interference was 6.5%,the intraday and daytime precision were 0.39%and 0.52%,respectively,the accuracy was 102.5%-107.5%,and the relative standard deviation(RSD)was less than 6.5%.

关 键 词：数字图像比色法 2 4-二硝基苯肼 肉桂油 醛类化合物 

分 类 号：TQ35[化学工程]