纤维蛋白原样蛋白2对EBV感染的T细胞免疫应答的影响研究  

Effect of fibrinogen-like protein 2 on immune response of EBV-infected T lymphocyte

作　　者：洪玉珍 李敏 程冰 刘跃平 刁波 HONG Yuzhen;LI Min;CHENG Bing;LIU Yueping;DIAO Bo(School of Medicine,Medical Department,Wuhan University of Science and Technology,Wuhan 430065,China;Basic Medical Laboratory,General Hospital of Central Theater Command,Wuhan 430070,China;Hubei Key Laboratory of Central Nervous System Tumor and Intervention,Wuhan 430070,China;Department of Disease Prevention and Control,General Hospital of Central Theater Command,Wuhan 430070,China)

机构地区：[1]武汉科技大学医学部医学院,430065 [2]中部战区总医院基础医学实验室,武汉430070 [3]中部战区总医院疾病预防控制科,武汉430070 [4]中枢神经系统肿瘤发生与干预湖北省重点实验室,武汉430070

出　　处：《免疫学杂志》2025年第1期16-23,共8页Immunological Journal

基　　金：湖北省自然科学基金(2023AFA093)。

摘　　要：目的本研究旨在探讨FGL2对EBV感染的T细胞免疫应答的影响,包括T细胞的活化、增殖、耗竭状态以及细胞因子谱的变化。方法使用不同感染复数的EBV感染原代T淋巴细胞,通过RT-qPCR、Western blot、ELISA、CCK8以及流式细胞术等方法,检测FGL2在T细胞中的表达及T细胞的活化、增殖、耗竭和细胞因子水平。进一步通过敲低和过表达FGL2,观察FGL2对EBV感染的T细胞免疫应答的具体影响。结果FGL2在EBV感染的T细胞中表达显著上调(P<0.05)。同时,EBV感染后T细胞出现活化(P<0.001)、增殖(P<0.001)和耗竭(P<0.01)水平升高。与T细胞+EBV组相比,T细胞+EBV+FGL2过表达组T细胞的耗竭水平更高(P<0.01),活化(P<0.05)和增殖(P<0.05)程度更低,促炎性细胞因子水平降低(P<0.05),抑炎性细胞因子水平升高(P<0.05);而T细胞+EBV+FGL2敲低组则表现出相反的趋势,即T细胞的活化(P<0.01)、增殖水平更高(P<0.05),耗竭程度更低(P<0.01),促炎性细胞因子水平升高(P<0.05),抑炎性细胞因子水平降低(P<0.05)。结论FGL2在EBV感染中能抑制T细胞的活化和增殖,增加T细胞的耗竭程度,同时抑制促炎性细胞因子的释放并促进抑炎性细胞因子的释放,从而调控T细胞的免疫应答。Objective This study aims to investigate the effects of FGL2 on the immune response of EBV-infected T cells,including their activation,proliferation,exhaustion,and cytokine profile changes.Methods Primary T cells were infected with EBV at different multiplicities of infection(MOI).Expression of FGL2 in T cells,as well as T-cell activation,proliferation,exhaustion,and cytokine levels,were detected using RT-qPCR,Western blot,ELISA,CCK8,and flow cytometry(FCM),respectively.Further experiments involving FGL2 knockdown and overexpression were conducted to elucidate its specific regulatory role in EBV-infected T cells.Results FGL2 expression was significantly upregulated in EBV-infected T cells(P<0.05).EBV infection also induced enhanced T cell activation(P<0.001),proliferation(P<0.001),and exhaustion(P<0.01).Compared to the T cells+EBV group,the T cells+EBV+FGL2 overexpression group exhibited higher exhaustion levels(P<0.01),reduced activation(P<0.05)and proliferation(P<0.05),decreased pro-inflammatory cytokine levels(P<0.05),and increased anti-inflammatory cytokine levels(P<0.05).Conversely,the T cells+EBV+FGL2 knockdown group demonstrated the opposite trends,with elevated activation(P<0.01),proliferation(P<0.05),pro-inflammatory cytokine levels(P<0.05),and reduced exhaustion(P<0.01)and antiinflammatory cytokine levels(P<0.05).Conclusion FGL2 suppresses T cell activation and proliferation,exacerbates T cell exhaustion,inhibits pro-inflammatory cytokine release,and promotes anti-inflammatory cytokine secretion during EBV infection,thereby modulating the immune response of T cells.

关 键 词：EB病毒 纤维蛋白原样蛋白2 T细胞耗竭 T细胞活化 感染复数 

分 类 号：R373[医药卫生—病原生物学]