帕金森病患者血清LncRNA-PART1、LncRNA-SNHG14水平与疾病分期、认知障碍、运动功能的相关性研究  

Correlation between serum levels of LncRNA-PART1 and LncRNA-SNHG14 with disease stage,cognitive impairment and motor function in patients with Parkinson's disease

作　　者：高聚[1] 王佳君[1] 夏雪彬 吴锐 江昕 肖展翅 GAO Ju;WANG Jiajun;XIA Xuebin;WU Rui;JIANG Xin;XIAO Zhanchi(Department of Neurology,Huanggang Central Hospital(Dabie Mountain Area Medical Center),Huanggang,Hubei 438000,China)

机构地区：[1]黄冈市中心医院(大别山区域医疗中心)神经内科,湖北黄冈438000

出　　处：《国际检验医学杂志》2025年第8期943-947,954,共6页International Journal of Laboratory Medicine

基　　金：湖北省中央引导地方科技发展专项项目(2022BCE047)。

摘　　要：目的探讨帕金森病(PD)患者血清长链非编码RNA(LncRNA)前列腺雄激素调控转录物1(PART1)、LncRNA-核仁核糖核酸宿主基因14(SNHG14)水平与病情分期、认知障碍、运动功能的相关性。方法选取2021年1月至2023年12月该院神经内科收治的PD患者100例(PD组)和100例体检健康者(对照组),PD患者根据Hoehn-Yahr分级分为早期组(1.0~2.5级,20例)、中期组(3.0级,48例)、晚期组(4.0~5.0级,32例),根据蒙特利尔认知评估量表(MoCA)评分为正常认知组(MoCA评分≥26分,33例)、PD-轻度认知障碍组(MoCA评分21~<26分,46例)、PD痴呆组(MoCA评分<21分,21例),根据帕金森病统一评分量表(UPDRS)-Ⅲ评分分为轻度运动障碍组(0~15分,29例)、中度运动障碍组(>15~40分,46例)、重度运动障碍组(>40~56分,25例)。采用实时荧光定量PCR检测血清LncRNA-PART1、LncRNA-SNHG14水平。通过Spearman法分析血清LncRNA-PART1、LncRNA-SNHG14水平与PD患者Hoehn-Yahr分期、MoCA评分、UPDRS-Ⅲ评分的相关性。结果PD组血清LncRNA-PART1水平低于对照组(P<0.05),LncRNASNHG14水平高于对照组(P<0.05)。中期组、晚期组血清LncRNA-PART1水平低于早期组(P<0.05),LncRNA-SNHG14水平高于早期组(P<0.05),且晚期组血清LncRNA-PART1水平低于中期组(P<0.05),LncRNA-SNHG14水平高于中期组(P<0.05)。PD-轻度认知障碍组、PD痴呆组血清LncRNA-PART1水平低于正常认知组(P<0.05),LncRNA-SNHG14水平高于正常认知组(P<0.05),且PD痴呆组血清LncRNAPART1水平低于PD-轻度认知障碍组(P<0.05),LncRNA-SNHG14水平高于PD-轻度认知障碍组(P<0.05)。中度运动障碍组、重度运动障碍组血清LncRNA-PART1水平低于轻度运动障碍组(P<0.05),LncRNA-SNHG14水平高于轻度运动障碍组(P<0.05),且重度运动障碍组血清LncRNA-PART1水平低于中度运动障碍组(P<0.05),LncRNA-SNHG14水平高于中度运动障碍组(P<0.05)。Spearman法结果显示,血清LncRNA-PART1水平与PD患者Hoehn-Yahr分期、UPDRS-Ⅲ评分呈负相关(P<Objective To investigate the correlation between serum levels of long non-coding RNA(LncRNA)-prostate androgen regulated transcript 1(PART1),LncRNA-nucleolar ribonucleic acid host gene 14(SNHG14)and disease stage,cognitive impairment and motor function in patients with Parkinson's disease(PD).Methods A total of 100 PD patients(PD group)who admitted to the Department of Neurology in the hospital from January 2021 to December 2023 and 100 healthy subjects(control group)who underwent the physical examination during the same period of time were selected.According to Hoehn-Yahr staging,PD patients were divided into early stage group(grade 1.0-2.5,20 cases),middle stage group(grade 3.0,48 cases)and late stage group(grade 4.0-5.0,32 cases).According to the Montreal Cognitive Assessment(Mo-CA)score,the patients were divided into normal cognitive group(MoCA score≥26 points,33 cases),PD-mild cognitive impairment group(MoCA score 21-<26 points,46 cases)and PD dementia group(MoCA score<21 points,21 cases).According to the Unified Parkinson's Disease Rating Scale(UPDRS)-Ⅲscore,the patients were divided into mild dyskinesia group(0-15 points,29 cases),moderate dyskinesia group(>15-40 points,46 cases)and severe dyskinesia group(>40-56 points,25 cases).Real-time fluorescence quantitative PCR was used to detect serum LncRNA-PART1 and LncRNA-SNHG14 levels.Spearman method was used to analyze the correlation between serum LncRNA-PART1,LncRNA-SNHG14 levels and Hoehn-Yahr staging,MoCA score and UPDRS-Ⅲscore in PD patients.Results The level of serum LncRNA-PART1 in PD group was lower than that in control group(P<0.05),and the level of LncRNA-SNHG14 was higher than that in control group(P<0.05).The serum levels of LncRNA-PART1 in the middle stage group and late stage groups were lower than those in the early stage group(P<0.05),and the levels of LncRNA-SNHG14 were higher than those in the early stage group(P<0.05).In addition,the serum level of LncRNA-PART1 in the late stage group was lower than that in the middle stage group(P<0.

关 键 词：帕金森病 长链非编码RNA-前列腺雄激素调控转录物1 长链非编码RNA-核仁核糖核酸宿主基因14 病情分期 认知障碍 运动功能 

分 类 号：R446.1[医药卫生—诊断学]