甘蓝型油菜品种中双11根肿病抗性和除草剂抗性的遗传改良  

Genetic improvement of resistance to clubroot and herbicide of Brassica napus cultivar Zhongshuang11

作　　者：杨静[1] 王朝阳 张晓晖 徐义[1] 王鹏飞 宋易先 洪登峰[1] 杨光圣[1,2] YANG Jing;WANG Zhao-yang;ZHANG Xiao-hui;XU Yi;WANG Peng-fei;SONG Yi-xian;HONG Deng-feng;YANG Guang-sheng(National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430070,China;Wuhan Liannong Seed Technology Co.,Ltd.,Wuhan 430070,China)

机构地区：[1]华中农业大学作物遗传改良全国重点实验室,湖北武汉430070 [2]武汉联农种业科技有限责任公司,湖北武汉430070

出　　处：《中国油料作物学报》2025年第2期302-310,共9页Chinese Journal of Oil Crop Sciences

基　　金：国家重点研发计划(2022YFF1002200);科技创新2030重大项目(2022ZD0400801)。

摘　　要：根肿病和草害严重威胁油菜的产量和品质。为选育抗根肿病(clubroot-resistant,CR)和抗除草剂(herbicide-resistant,HR)的油菜品种,通过分子标记辅助选择聚合育种策略将抗根肿病位点CRb和PbBa8.1、抗除草剂位点ALS1R和ALS3R导入油菜常规品种中双11(ZS11)中,获得3个改良株系ZS11CR(CRb+PbBa8.1)、ZS11HR(ALS1R+ALS3R)和ZS11CHR(CRb+PbBa8.1+ALS1R+ALS3R)。利用根肿菌4号生理小种(湖北枝江)和噻吩磺隆除草剂(45 g a.i.ha^(-1))对ZS11CR、ZS11HR和ZS11CHR的抗性进行评价,结果表明:ZS11CR、ZS11CHR对4号生理小种抗性达到免疫水平,ZS11HR、ZS11CHR对噻吩磺隆除草剂抗性显著。田间农艺性状调查结果表明,ZS11CR、ZS11HR和ZS11CHR的株高较ZS11一定程度增加,而在开花期、分枝数、主花序角果数、角果长、每角果粒数、千粒重等性状上没有显著差异。本研究获得了3个改良株系,其中ZS11CR具有根肿病抗性、ZS11HR具有除草剂抗性、ZS11CHR兼具根肿病抗性和除草剂抗性,这些改良株系不仅目标性状得到了改良,同时维持了ZS11的优良农艺性状,具有一定的应用潜力。Clubroot and weeds are serious threats to rapeseed production in China.To develop clubroot-resistant(CR)and herbicide-resistant(HR)cultivars,we developed 3 improved lines ZS11CR(CRb+PbBa8.1),ZS11HR(ALS1R+ALS3R)and ZS11CHR(CRb+PbBa8.1+ALS1R+ALS3R)by pyramiding of 2 clubroot resistance locus(CRb+PbBa8.1)and 2 herbicide-resistance locus(ALS1R+ALS3R)into rapeseed cultivar Zhongshuang 11(ZS11)with marker-assisted selection.Clubroot and herbicide resistance of the improved lines(ZS11CR,ZS11HR and ZS11CHR)was evaluated by using clubroot pathotype 4(Zhijiang,Hubei Province)and thifensulfuron herbicide(45 g a.i.ha^(-1)),respectively.According to the results,ZS11CR and ZS11CHR were immune to pathotype 4,and ZS11HR and ZS11CHR exhibited highly resistance against thifensulfuron herbicide.In agronomic traits,ZS11CR,ZS11HR and ZS11CHR showed higher plant heights than ZS11,but no significant differences on the following traits as flowering time,number of the branch,siliques of the main inflorescence,silique length,number of seeds per silique and thousand seed weight.The enhanced ZS11CR line exhibited strong resistance against clubroot,while ZS11HR demonstrated high resistance against herbicide.ZS11CHR displayed resistance against both clubroot and herbicide.Additionally,these improved lines maintain outstanding agronomic characteristics,providing some potential for utilization in rapeseed breeding.

关 键 词：甘蓝型油菜 中双11 根肿病抗性 除草剂抗性 噻吩磺隆 聚合育种 

分 类 号：S565.4[农业科学—作物学]