强抗寒甘蓝型冬油菜愈伤组织体系构建及ROS信号传导规律探究  

Callus system construction and ROS signal transduction mechanism of cold resistant winter rape

作　　者：祁伟亮 孙万仓[4] 邓素敏 高雪梅 马骊[4] 李妮[1] 张雅 杨财容 QI Wei-liang;SUN Wan-cang;DENG Su-min;GAO Xue-mei;MA Li;LI Ni;ZHANG Ya;YANG Cai-rong(School of Agriculture and Bioengineering,Longdong University,Qingyang 745000,China;Gansu Dryland Research Center of Winter Wheat Germplasm Innovation and Application Engineering,Qingyang 745000,China;Collaborative Innovation Center for Longdong Dryland Crop Germplasm Improvement and Industrialization,Qingyang 745000,China;College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China;Chengdu Normal University,Chengdu 611130,China)

机构地区：[1]陇东学院农业与生物工程学院,甘肃庆阳745000 [2]甘肃省旱地冬小麦种质创新与应用工程研究中心,甘肃庆阳745000 [3]陇东旱地作物种质改良及产业化协同创新中心,甘肃庆阳745000 [4]甘肃农业大学农学院,甘肃兰州730070 [5]成都师范学院,四川成都611130

出　　处：《中国油料作物学报》2025年第2期311-318,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(32360455);陇东学院博士基金项目(XYBYZK2107);甘肃省高等学校创新基金项目(2022B-209)。

摘　　要：为了解活性氧(ROS)介导甘蓝型冬油菜愈伤组织生长发育中的作用机制,以甘蓝型冬油菜16VHNTS309为材料,分别以其下胚轴和幼叶作为外植体,构建甘蓝型油菜的再生体系,并对愈伤组织进行(ROS)O_(2)^(-)信号定位。结果表明:幼叶和下胚轴分别在MS+1 mg/L和1.5 mg/L 2,4-D培养基预培养7 d后,再以MS+3.0 mg/L 6-BA+0.2 mg/L 2,4-D+3 mg/L AgNO_(3)为最优培养基进行芽的诱导,最后以MS+0.2 mg/L NAA为生根培养基,可得到较好的甘蓝型油菜再生苗。ROS(O_(2)^(-))信号定位结果表明:具有分裂能力的愈伤组织细胞、顶端分生组织细胞和叶或茎边缘组织细胞会检测到大量的ROS(O_(2)^(-))信号,而已完成芽体分化或未形成愈伤组织的细胞周围,未检测到ROS(O_(2)^(-))信号,该研究结果进一步说明ROS(O_(2)^(-))信号在细胞分裂过程中扮演着重要角色。To understand the mechanism of reactive oxygen species(ROS)in mediating callus development of winter rapeseed(Brassica napus L.),hypocotyl and young leaf of Brassica napus var.16VHNTS309 was used as explants to construct a regeneration system of B.napus.Through observation of reactive oxygen species of O_(2)^(-)signal localization in callus,results showed an optimized system of young leaves and hypocotyls pre culture on MS+1 mg/L or 1.5 mg/L 2,4-D medium for 7 days,respectively.It was followed by MS+3.0 mg/L 6-BA+0.2 mg/L 2,4-D+3 mg/L AgNO_(3) used as the optimal medium for bud induction.Finally,MS+0.2 mg/L NAA could be used as the rooting medium to obtain good regenerated seedlings of B.napus.Results of ROS(O_(2)^(-))signal localization showed that a large amount of ROS(O_(2)^(-))signal was detected in callus cells,meristem cells and cells at the edge of leaves or stems,while ROS(O_(2)^(-))signal was not detected around cells that had completed bud differentiation or did not form calli,which further indicated that ROS(O_(2)^(-))signal played an important role in cell division.

关 键 词：甘蓝型油菜 幼叶 下胚轴 愈伤组织 活性氧 信号传导 

分 类 号：S565.4[农业科学—作物学]