大豆芽期耐旱性QTL定位及相关基因挖掘  

QTL mapping and related gene mining of drought tolerance at germination stage in soybean

作　　者：谢建国 王明亮[1] 张云峰[1] 孟凡凡[1] 郑宇宏[1] 李广 孙星邈[1] 范旭红 杨振宇[1] 王曙明[1] 蒋洪蔚 XIE Jian-guo;WANG Ming-liang;ZHANG Yun-feng;MENG Fan-fan;ZHENG Yu-hong;LI Guang;SUN Xing-miao;FAN Xu-hong;YANG Zhen-yu;WANG Shu-ming;JIANG Hong-wei(Jilin Academy of Agricultural Sciences/China Agricultural Science and Technology Northeast Innovation Center/Soybean Research Institute,Changchun 130033,China)

机构地区：[1]吉林省农业科学院/中国农业科技东北创新中心/大豆研究所,吉林长春130033

出　　处：《中国油料作物学报》2025年第2期328-337,共10页Chinese Journal of Oil Crop Sciences

基　　金：国家现代农业产业技术体系(CARS-04-PS08);吉林省极早熟区高油高产大豆新品种培育(2023ZD0403203-01)。

摘　　要：大豆(Glycine max)是世界范围内重要的粮食作物和油料作物。近年来我国东北地区春播期间干旱天气频发,导致大豆种子发芽质量差、出苗率低。为解析芽期耐旱遗传机制,本研究采用20%的聚乙二醇(PEG-6000)模拟干旱胁迫对201份染色体片段代换系材料进行芽期耐旱性鉴定。以干旱条件下发芽势、发芽率、发芽指数、相对发芽势、相对发芽率、相对发芽指数与干旱伤害率为鉴定指标,聚类分析得到19份耐旱材料、131份中间型材料和51份敏感材料。采用完备区间作图方法(ICIM)法对各鉴定指标进行QTL定位,共定位到3个QTL,其中qDT-4-1与qDT-15-2在多个性状中被定位到。在qDT-4-1与qDT-15-2中共注释到17个基因,通过双亲间氨基酸序列比对和实时荧光定量分析,推测基因Glyma.15G196400可能与大豆芽期耐旱相关。利用258份大豆种质资源对候选基因Glyma.15G196400进行单倍型分析,得到2个优异单倍型Hap-1和Hap-2。两个优异单倍型Hap-1和Hap-2在启动子区域-2001bp处存在G-A的变异,引起CAAT-box变异,两个优异单倍型的发芽率、发芽势和发芽指标分别在0.05水平上差异显著,说明该候选基因在大豆群体中具有广泛的适用性。本研究将为大豆芽期耐旱育种提供优质材料以及为芽期耐旱遗传机制解析提供理论指导。In recent years,drought weather has occurred frequently during spring sowing in Northeast China,resulting in poor germination quality and low emergence rate of soybean seeds.In this study,20%polyethylene glycol(PEG-6000)was used to simulate drought stress to identify drought tolerance of 201 chromosome segment substitution line populations at germination stage.Germination potential,germination rate,germination index,relative germination potential,relative germination rate,relative germination index and drought damage rate were used as identification indexes.Cluster analysis obtained 19 drought tolerant materials,131 intermediate materials,and 51 sensitive materials.Inclusive composite interval mapping(ICIM)method was used to locate QTLs for each germination index,and a total of 3 QTLs were located,of which qDT-4-1 and qDT-15-2 were located in multiple traits.A total of 17 genes were annotated in qDT-4-1 and qDT-15-2.Based on amino acid sequence alignment and realtime fluorescence quantitative analysis between parents,it was speculated that gene Glyma.15G196400 might be related to drought tolerance at germination stage.Haplotype analysis of candidate gene Glyma.15G196400 was performed using 258 soybean germplasm resources,and 2 elite haplotypes Hap-1 and Hap-2 were obtained.These 2 haplotypes had a G-A mutation at-2001 bp,which caused a mutation of CAAT-box promoter.The germination rate,germination potential and germination index of Hap-1 and Hap-2 were significantly different at 0.05 level,in dicating that the candidate gene had a wide range of applicability in the soybean population.

关 键 词：大豆芽期耐旱 资源鉴定 QTL定位 候选基因 单倍型 

分 类 号：S565.1[农业科学—作物学]