花生CC-NBS-LRR家族基因鉴定及在青枯病和网斑病胁迫下的表达分析  

Genome-wide identification and analysis of CC-NBS-LRR family in response to bacterial wilt and web blotch in peanut

作　　者：汪晓 齐飞艳 孙子淇 郑峥 徐静 王娟 韩锁义 黄冰艳 董文召 张新友 WANG Xiao;QI Fei-yan;SUN Zi-qi;ZHENG Zheng;XU Jing;WANG Juan;HAN Suo-yi;HUANG Bing-yan;DONG Wen-zhao;ZHANG Xin-you(Shenyang Agricultural University,Shenyang 110866,China;The Shennong Laboratory,Zhengzhou 450002,China;Institute of Crop Molecular Breeding,Henan Academy of Agricultural Sciences/National Innovation Centre for Bio-breeding Industry/Key Laboratory of Oil Crops in Huanghuaihai Plains,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement/Henan Biological Breeding Center CO.,Ltd.,Zhengzhou 450002,China)

机构地区：[1]沈阳农业大学,辽宁沈阳110866 [2]神农种业实验室,河南郑州450002 [3]河南省作物分子育种研究院/国家生物育种产业创新中心/农业农村部黄淮海油料作物重点实验室/河南省油料作物遗传改良重点实验室/河南生物育种中心有限公司,河南郑州450002

出　　处：《中国油料作物学报》2025年第2期344-355,共12页Chinese Journal of Oil Crop Sciences

基　　金：神农种业实验室一流课题(SN01-2022-03);河南省农业科学院杰出青年科技基金(2024JQ06);河南省农科院自主创新项目(2023ZC092);财政部和农业农村部:国家现代农业产业技术体系资助(CARS-13);河南省花生产业技术体系(S2012-5);优质抗病花生新品种选育(2024TD01)。

摘　　要：为明确花生基因组中CC-NBS-LRR(CNL)的家族成员及其功能,对其基因家族成员进行生物信息学分析和表达模式分析,研究花生如何响应病害胁迫。以花生基因组序列为参考,利用Pfam、HMMER、NCBI和Coiled-Coil等软件和在线网站筛选,共鉴定得到85个AhCNL家族成员,分布在20条染色体中的15条上,以A02和A12染色体上分布最多,并且大多成簇存在于染色体末端;系统进化树分析表明其可分为4个亚族,每个亚族中的基因成员保守性较高;共线性分析表明在物种内有16对基因存在共线性关系,物种间分析表明相比于拟南芥和苜蓿,花生与大豆的CNL具有较高的同源性;启动子上含有大量与激素相关和抗病相关的顺式作用元件;大部分CNL家族基因在病菌入侵前后均不表达,少部分基因受到病菌诱导后表达量有变化。In order to identify the CC-NBS-LRR(CNL)family members and their functions in peanut genome,tbioinformatics analysis and expression pattern of the gene family members were carried out to lay a foundation for peanut responds to disease stress.Using peanut genome sequences as reference,85 AhCNL family members were identified using Pfam,HMMER,NCBI and Coiled Coil software and online sites.AhCNL genes are distributed on 15 out of 20 chromosomes,mainly on A02 and A12.Most of them exist in clusters at chromosomes end.Phylogenetic tree analysis showed that AhCNL genes can be divided into 4 subfamilies,and the gene members in each subfamily are highly conserved.Collinearity analysis showed that there were 16 pairs of genes in the species,and inter-species analysis showed that CNL of peanut and Glycine max had higher homology than those of Arabidopsis thaliana and Medicago sativa.The promoters of AhCNL genes contain a large number of cis-acting elements related to hormone and disease resistance.Most CNL family genes were not expressed before and after being invaded by pathogens,with a few genes changed after being induced.

关 键 词：花生 CC-NBS-LRR 青枯病 网斑病 表达分析 

分 类 号：S565.2[农业科学—作物学]