花生疮痂病菌次生代谢基因簇特异性转录因子挖掘及EaPSTF1生物信息学分析  

Mining of secondary metabolite gene cluster-specific transcription factors and EaPSTF1 bioinformatics of Elsinoe arachidis

作　　者：张凌萱 朴静子 刘丹[1] 郝婧文 李自博 周如军[1] ZHANG Ling-xuan;PIAO Jing-zi;LIU-Dan;HAO Jing-wen;LI Zi-bo;ZHOU Ru-jun(College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China)

机构地区：[1]沈阳农业大学,辽宁沈阳110866

出　　处：《中国油料作物学报》2025年第2期356-362,共7页Chinese Journal of Oil Crop Sciences

基　　金：辽宁省自然科学基金(2022-MS-264);辽宁省教育厅面上项目(JYTMS20231291)。

摘　　要：痂囊腔菌素(Elsinchrome,ESC)是花生疮痂病菌产生的一种具有光敏活性的苝醌类真菌毒素,为病菌的毒力因子。为了进一步揭示ESC生物合成的调控网络,在全基因组测序分析基础上,开展了病菌次生代谢基因簇特异性转录因子的挖掘、EaPSTF1基因克隆、生物信息学和表达分析。结果表明,病菌次生代谢基因簇上共有3个特异性转录因子,其中EaPSTF1全长1305 bp,含有一个完整的开放阅读框,编码长度为434个氨基酸的蛋白质,分子量110.58 kD,理论等电点4.94,亚细胞定位细胞核中,是一个以α-螺旋为主的亲水性蛋白,含有GAL4和AFLR两个Zn(II)2Cys6型结构域。RT-qPCR定量分析表明,EaPSTF1表达模式与毒素累积趋势基本一致,EaPSTF1可能参与ESC毒素的生物合成调控。Elsinchrome(ESC)is a photosensitive perylene quinone mycotoxin produced by Elsinoëarachidis,which acts as the virulence factor of the pathogen.For deep understanding of the regulatory network governing ESC biosynthesis,we conducted a comprehensive analysis including identification of transcription factors specific to the secondary metabolism gene cluster,EaPSTF1 gene clone,bioinformatics and expression analysis based on wholegenome sequencing.The findings revealed the presence of 3 distinct transcription factors within the pathogen's secondary metabolism gene cluster.Among them,EaPSTF1 had a length of 1305 bp comprising a complete open reading frame,encoding a 110.58 kD-protein with 434 amino acids.And its theoretical isoelectric point was 4.94.Primarily located in cell nucleus,EaPSTF1 is a hydrophilic protein dominated byα-helices.It contains 2 Zn(II)2Cys6-type domains,GAL4 and AFLR.RT-qPCR analysis demonstrated a consistent expression pattern of EaPSTF1 with toxin accumulation trend,indicating a potential involvement in regulation of ESC toxin biosynthesis.

关 键 词：花生疮痂病菌 特异性转录因子 基因克隆 生物信息学分析 次生代谢基因簇 

分 类 号：S565.2[农业科学—作物学]