BnaNF-YA2增强甘蓝型油菜渗透胁迫抗性的功能鉴定  

Functional characterization of BnaNF-YA2 in enhancing osmotic stress resistance in Brassica napus

作　　者：王高升 王佩瑶 孙念礼 王幼平[1] 方玉洁[1] WANG Gao-sheng;WANG Pei-yao;SUN Nian-li;WANG You-ping;FANG Yu-jie(College of Biological Science and Technology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学生物科学与技术学院,江苏扬州225009

出　　处：《中国油料作物学报》2025年第2期421-434,共14页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(32171969);江苏省农业科技自主创新资金(CX(22)3085)。

摘　　要：甘蓝型油菜是我国主要的食用植物油来源之一。核因子Y(NF-Y)是植物中非常重要的一类转录因子,其关键组分NF-YA通常与NF-YB/NF-YC二聚体形成三聚体发挥功能,在植物的生长发育和胁迫响应方面都有重要作用。对BnaNF-YA家族成员在甘蓝型油菜响应逆境胁迫中的功能进行分析,可以为培育具有优良抗逆性的油菜新材料提供理论依据。通过对BnaNF-YA2进行生物信息学分析和表达模式分析,创建过表达BnaNF-YA2的甘蓝型油菜,并对过表达植株进行逆境表型鉴定等方法分析BnaNF-YA2在参与油菜非生物胁迫应答中的功能。顺式元件分析结果表明,BnaNF-YA2启动子包含多个胁迫响应元件;表达分析结果显示,BnaNF-YA2受PEG、高温、低温处理诱导表达;过表达BnaNF-YA2改善了甘蓝型油菜在PEG和甘露醇处理条件下的生长状况。BnaNF-YA2正调控甘蓝型油菜对渗透胁迫的耐受性。Rapeseed(Brassica napus L.)is one of the main sources of edible vegetable oil in China.Nuclear factor Y(NF-Y)proteins comprise a very important transcription factor family in plants,and its key component NFYA usually functions with NF-YB/NF-YC dimer to form a trimer,which plays an important role in plant development and stress response.To better understand the function of BnaNF-YA family members in response to abiotic stress,and to provide a theoretical basis for cultivating rapeseed materials with improved stress resistance,we analyzed BnaNF-YA2 in response to abiotic stress by bioinformatics and expression patterns,by overexpressing BnaNF-YA2 in B.rapus and identifying their stress phenotypes.Identification results of cis-elements showed that the BnaNF-YA2 promoter region were rich in stress-related response elements.The expression analysis revealed that PEG,heat and low temperature induced BnaNF-YA2 expression.Results also indicated that BnaNF-YA2 overexpression might enhance the growth of B.napus under PEG and mannitol treatments during the post-germination stage.BnaNF-YA2 positively regulated the tolerance of B.napus to osmotic stress.

关 键 词：甘蓝型油菜 转录因子 NF-YA 渗透胁迫 表型鉴定 

分 类 号：S565.4[农业科学—作物学]