潼蒺藜对实验性干眼病大鼠泪腺相关炎症因子表达的影响  

Effect of Astragalus Pratensis on Expression of Lacrimal Gland Related Inflammatory Factors in Experimental Dry Eye Rats

作　　者：侯捷[1] 杨希玲 李日伦[1] 寇亚丽[1] 刘笑[1] 刘露 侯秋科[2] HOU Jie;YANG Xiling;LI Rilun;KOU Yali;LIU Xiao;LIU Lu;HOU Qiuke(Guangxi Health Vocational and Technical College,Nanning 530023,China;The First Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou 510405,China)

机构地区：[1]广西卫生职业技术学院,广西南宁530023 [2]广州中医药大学第一附属医院,广东广州510405

出　　处：《云南中医药大学学报》2025年第2期88-94,共7页Journal of Yunnan University of Chinese Medicine

基　　金：广西中青年科研能力提升项目(2022KY1393)。

摘　　要：目的探讨潼蒺藜对实验性干眼病大鼠泪腺相关炎症因子表达及NF-κB信号通路的作用与抗炎机制。方法本研究纳入35只成年健康雄性大鼠,随机分为5组:空白对照组(N)、模型对照组(M)、溶剂对照组(R)、阳性药对照组(Y)、潼蒺藜给药组(T),每组7只大鼠。N组不做任何处理;M组、R组、Y组、T组均进行实验性干眼病模型构建,造模第1天开始分组给药,给药周期为14 d,用药结束后通过角膜荧光素染色(fluorescein staining,FL)、泪膜破裂时间(break-up time,BUT)、泪液分泌试验(schirmer I-test,SIT)对各组药效进行评价,ELISA法检测各组实验大鼠血清中泪腺相关炎症因子TNF-α、IL-1α和IL-6水平,免疫组化和PCR法检测各组实验大鼠泪腺组织中NF-κB信号通路主要因子(IKK-α、IKK-β、Iκ-B、NF-κB-p65)表达的改变,了解大鼠泪腺相关炎症因子及通路表达情况。结果FL、BUT、SIT的检测结果显示,潼蒺藜可改善干眼病大鼠的的泪液分泌量,延长角膜破裂时间;ELISA法检测结果显示,潼蒺藜可降低干眼病大鼠的血清泪腺相关炎症因子TNF-α、IL-1α和IL-6的表达水平;免疫组化和PCR法检测结果显示,潼蒺藜可降低NF-κB信号通路主要因子的表达水平。结论潼蒺藜可通过抑制NF-κB信号通路,从而降低泪腺相关炎症因子的表达水平,起到缓解干眼病大鼠泪腺炎症反应的作用。Objective To investigate the effect and anti-inflammatory mechanism of astragalus pratensison expression of lacrimal gland related inflammatory factors and NF-κB signaling pathway in experimental dry eye disease rats.Methods In this study,35 adult healthy male rats were randomly divided into 5 groups:blank control group(N),model control group(M),solvent control group(R),positive drug control group(Y),and astragalus pratensis administration group(T),with 7 rats in each group.The N group does not do any processing.Experimental dry eye models were constructed in groups M,R,Y and T,and drug administration was started in groups on the first day of modeling,and the administration cycle was 14 days.Fluorescein staining(FL),break-up time(BUT)and tear secretion Schirmer I-test(SIT)were evaluated.The levels of TNF-α,IL-1αand IL-6 in serum of rats in each group were detected by ELISA,and the expression of major factors of NF-κB signaling pathway(IKK-α,IKK-β,Iκ-B,NF-κB-P65)in lacrimal tissues of rats in each group were detected by immunohistochemistry and PCR to investigate the expression of lacrimal gland related inflammatory factors and pathways in rat.Results FL,BUT and SIT showed that astragalus pratensis can improve tear secretion and prolong corneal rupture time in rats with dry eye disease.ELISA results showed that astragalus pratensis could decrease the expression levels of TNF-α,IL-1αand IL-6 in the serum of rats with dry eye disease.Immunohistochemical and PCR assay results showed that astragalus pratensis could reduce the expression level of major factor of NF-κB signaling pathway.Conclusion Astragalus pratensis can reduce the expression of lacrimal inflammatory factors by inhibiting NF-κB signaling pathway,and play a role in alleviating the inflammatory reaction of lacrimal gland in rats with dry eye disease.

关 键 词：潼蒺藜 干眼病 泪腺 NF-ΚB信号通路 炎症因子 

分 类 号：R285.5[医药卫生—中药学]